IL-33介导Treg细胞功能参与布鲁氏菌病免疫调节机制研究  

IL-33 mediates the immune regulation mechanism of brucellosis by regulating Treg cell function

作　　者：李智伟[1] 早克然·阿力肯 王玲玲[1] 贾金彤 李书灵 朱晓玉 王倩 卢佩佩[1] 王昌敏[1] LI Zhiwei;Zaokeran·Aliken;WANG Lingling;JIA Jintong;LI Shuling;ZHU Xiaoyu;WANG Qian;LU Peipei;WANG Changmin(Clinical Laboratory Center,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830001,China;Department of Laboratory Medicine,Shawan People′s Hospital,Shawan,Xinjiang 832100,China;School of Medicine,Shihezi University,Shihezi,Xinjiang 832003,China;Graduate School of Xinjiang Medical University,Urumqi,Xinjiang 830011,China)

机构地区：[1]新疆维吾尔自治区人民医院临床检验中心,新疆乌鲁木齐830001 [2]沙湾市人民医院检验科,新疆沙湾832100 [3]石河子大学医学院,新疆石河子832003 [4]新疆医科大学研究生院,新疆乌鲁木齐830011

出　　处：《国际检验医学杂志》2024年第18期2184-2188,2196,共6页International Journal of Laboratory Medicine

基　　金：新疆维吾尔自治区自然科学基金杰出青年科学基金项目(2022D01E30);新疆维吾尔自治区“天山英才”培养计划项目(2022TSYCCX0102);新疆维吾尔自治区科技支疆计划项目(2022E02118);新疆维吾尔自治区自然科学基金面上项目(2023D01C83,2023D01C84)。

摘　　要：目的分析白细胞介素(IL)-33和调节性T(Treg)细胞在布鲁氏菌病(简称布病)患者中的变化特点,验证IL-33对Treg细胞的调节作用,阐明布病中IL-33对Treg细胞调节的免疫机制。方法采集2021年1-12月在新疆维吾尔自治区人民医院就诊的39例布病患者(布病组)和同期体检的42例健康对照者(健康对照组)的外周血,使用AimPlex试剂盒检测血清IL-33水平,使用流式细胞术检测Treg细胞比例,提取外周血单个核细胞(PBMC)进行体外培养试验观察IL-33刺激后和阻断后叉头框蛋白P3(Foxp3)比例和mRNA表达水平变化。结果与健康对照组比较,布病组IL-33水平与Treg细胞比例均显著增高,差异均有统计学意义(P<0.05)。体外试验显示,两组PBMC在IL-33刺激后Foxp3比例和mRNA表达水平显著增高,IL-33阻断后Foxp3比例和mRNA表达水平显著回落,差异均有统计学意义(P<0.001)。结论布病患者IL-33和Treg细胞显著增多,IL-33促进Treg细胞的免疫功能,阻断IL-33有望成为免疫治疗布病的潜在靶点。Objective To investigate the characteristics of changes in interleukin(IL)-33 and regulatory T(Treg)cells in brucellosis,to verify the regulatory effect of IL-33 on Treg cells,so as to clarify the immune mechanism of IL-33 on Treg cells in brucellosis.Methods The peripheral blood of 39 patients with brucellosis treated in the People′s Hospital of Xinjiang Uygur Autonomous Region from January to December 2021(the brucellosis group)and 42 healthy controls(the healthy control group)who underwent physical examination during the same period were collected.The serum IL-33 level was detected by AimPlex kit,and the proportion of Treg cells was detected by flow cytometry.Peripheral blood mononuclear cell(PBMC)was extracted and cultured in vitro to observe the proportion and mRNA expression levels of forkhead box protein P3(Foxp3)after stimulation and blocking of IL-33.Results Compared with the healthy control group,the level of IL-33 and the proportion of Treg cells in brucellosis group were significantly increased,with statistical significance(P<0.05).In vitro tests showed that the Foxp3 proportion and mRNA expression level of PBMC in the two groups were significantly increased after IL-33 stimulation,and significantly decreased after IL-33 blocking,with statistical significance(P<0.001).Conclusion IL-33 and Treg cells increased significantly in brucellosis patients,and IL-33 promoted the immune function of Treg cells.Blocking IL-33 is expected to be a potential target for immunotherapy of brucellosis.

关 键 词：布鲁氏菌病 白细胞介素-33 调节性T细胞 免疫机制 

分 类 号：R446.1[医药卫生—诊断学] R181.32[医药卫生—临床医学]