血清LECT2、TL1A水平对特应性皮炎患儿病情的诊断价值  

Diagnostic value of serum LECT2 and TL1A levels in children with atopic dermatitis

作　　者：赵瑞雪 刘岩[1] 凌琬茗[1] 刘晓霞 陈银肖 ZHAO Ruixue;LIU Yan;LING Wanming;LIU Xiaoxia(Department of Dermatology,Hebei Children′s Hospital,Shijiazhuang,Hebei 050000,China)

机构地区：[1]河北省儿童医院皮肤科,河北石家庄050000

出　　处：《国际检验医学杂志》2024年第18期2240-2243,2249,共5页International Journal of Laboratory Medicine

基　　金：河北省2021年度医学科学研究课题计划(20211788)。

摘　　要：目的探究血清白细胞衍生趋化因子2(LECT2)、肿瘤坏死因子样配体1A(TL1A)水平检测对特应性皮炎(AD)患儿病情的诊断价值。方法选取2021年8月至2023年8月在该院就诊的83例AD患儿作为AD组,另选同期在该院体检的83例健康儿童作为对照组。采用酶联免疫吸附试验(ELISA)测定血清LECT2、TL1A表达水平,Spearman法分析血清LECT2、TL1A与AD积分指数(SCORAD)评分的相关性,多因素Logistic回归分析影响AD患儿病情严重程度的因素,受试者工作特征(ROC)曲线分析血清LECT2、TL1A水平对AD患儿病情严重程度的诊断效能。结果与对照组相比,AD组血清LECT2、TL1A表达水平均显著升高(P<0.05)。与低度组相比,中度组、重度组血清LECT2、TL1A、SCORAD评分、嗜酸性粒细胞直接计数(EOS)、总免疫球蛋白E(IgE)均依次显著升高(P<0.05)。根据Spearman相关性分析,AD组患儿血清LECT2、TL1A水平均与SCORAD评分呈正相关(r=0.776、0.693,均P<0.05)。根据多因素Logistic回归分析结果可以发现,血清LECT2、TL1A、EOS、总IgE是影响AD患儿病情严重程度的危险因素(P<0.05)。血清LECT2、TL1A二者联合诊断AD患儿病情严重程度的曲线下面积(AUC)优于血清LECT2、TL1A各自单独诊断(Z=2.249、1.989,P=0.025、0.047)。结论AD患儿血清LECT2、TL1A表达水平升高,与病情严重程度密切相关,二者联合可以更好地评估AD患儿病情严重程度。Objective To investigate the diagnostic value of serum levels of leukocyte cell derived chemotaxin 2(LECT2)and tumor necrosis factor ligand-related molecule-1A(TL1A)in children with atopic dermatitis(AD).Methods A total of 83 children with AD who were adimitted to the hospital from August 2021 to August 2023 were selected as the AD group,and 83 healthy children who underwent physical examination in the hospital during the same period were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was applied to measure the expression levels of LECT2 and TL1A in serum.Spearman method was applied to analyze the correlation between serum LECT2,TL1A,and AD integral index(SCORAD).Multivariate Logistic regression was applied to analyze the factors that affected the severity of AD in children.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic efficacy of serum LECT2 and TL1A levels on the severity of AD in children.Results Compared with the control group,the expression levels of LECT2 and TL1A in the serum of the AD group were significantly increased(P<0.05).Compared with the low-grade group,the serum LECT2,TL1A,SCORAD score,EOS and total IgE in the moderate and severe groups were significantly increased in turn(P<0.05).According to Spearman correlation analysis,the serum levels of LECT2 and TL1A in the AD group were positively correlated with the SCORAD score(r=0.776,0.693,both P<0.05).According to the results of multivariate Logistic regression analysis,serum LECT2、TL1A、EOS and total IgE were risk factors affecting the severity of AD in children(P<0.05).In addition,the combination of serum LECT2 and TL1A had the highest AUC for diagnosing the severity of AD in children,which was better than the individual diagnosis of serum LECT2 and TL1A(Z=2.249,1.989,P=0.025,0.047).Conclusion The elevated levels of serum LECT2 and TL1A in children with AD are closely related to the severity of the disease,and the combination of the two can better evaluate the severity of the disease in c

关 键 词：特应性皮炎 白细胞衍生趋化因子2 肿瘤坏死因子样配体1A 病情诊断 

分 类 号：R446.1[医药卫生—诊断学] R473.75[医药卫生—临床医学]