氯吡苯脲对台农1号芒果果实糖分及相关酶活性的影响  

Effects of CPPU on Sugar and Related Enzyme Activities of Mango Variety Tainong 1

作　　者：袁辉林 郭利军 郭彬 肖朝霞 刘金霞 YUAN Huilin;GUO Lijun;GUO Bin;XIAO Zhaoxia;LIU Jinxia(Hainan Education and Training Center for Agricultural Science and Technology,Haikou 571100,China;Institute of Tropical Fruit Trees,Hainan Academy of Agricultural Sciences,Haikou 571100,China)

机构地区：[1]海南省农民科技教育培训中心,海南海口571100 [2]海南省农业科学院热带果树研究所,海南海口571100

出　　处：《山西农业科学》2024年第5期124-129,共6页Journal of Shanxi Agricultural Sciences

基　　金：海南省自然科学基金项目(320RC753);海南省芒果产业技术体系(HNARS-07)。

摘　　要：为明确喷施不同质量浓度氯吡苯脲(CPPU)对芒果果实糖分代谢和相关酶活性的影响,探索CPPU对芒果蔗糖代谢的影响机制,以台农1号芒果植株为材料,分别于谢花后7、14 d喷施64 mg/L和256 mg/L的CPPU,以喷施清水为对照(CK),于成熟后测定果实淀粉、蔗糖、葡萄糖和果糖含量以及酸性转化酶(AI)、蔗糖磷酸合成酶(SPS)和腺苷二磷酸葡萄糖焦磷酸化酶(AGP)、结合态淀粉合成酶(GBSS)、淀粉分支酶(SBE)、可溶性淀粉合成酶(SSS)、总淀粉酶的活性,并分析果实糖组分与酶活性的相关性。结果表明,与CK相比,64 mg/L的CPPU处理对芒果果实的蔗糖、果糖和葡萄糖含量无显著影响;而256 mg/LCPPU处理则显著降低果糖和蔗糖含量,并显著提高淀粉含量。2个CPPU处理均显著提高芒果AGP的活性,同时降低SPS和AI的活性,并且256 mg/L的CPPU处理达到显著水平。果实糖组分与酶活性相关分析显示,淀粉含量与AGP活性呈正相关,蔗糖和果糖含量与SPS活性呈显著正相关,且蔗糖和果糖含量间呈极显著正相关;高质量浓度256 mg/L的CPPU处理降低果实糖组分含量的机理可能是通过降低SPS活性、降低蔗糖含量,同时通过提高AGP活性、增加果实淀粉含量,从而延缓果实淀粉向糖分转化进程。In order to clarify the effects of spraying different concentrations of Forchlorfenuron(CPPU)on sugar metabolism and related enzyme activities in mango plant,and to explore the influence mechanism of CPPU on mango sucrose metabolism,in this study,the fruit of mango variety Tainong 1 was used as the material,64 mg/L of CPPU and 256 mg/L of CPPU were sprayed at 7 d and 14 d after flower shedding,respectively,and spraying clear water was taken as the control(CK).After picking,the fruit starch,sucrose,glucose,and fructose content and acid conversion enzyme(AI),sucrose phosphate synthase(SPS)and adenosine diphosphate glucose pyrophosphorylase(AGP),bound starch synthetase(GBSS),starch branching enzyme(SBE),soluble starch synthase(SSS),total amylase activity were determined,then the correlation between the fruit sugar component and the enzyme activity was analyzed.The results indicate that compared to CK,64 mg/L concentration of CPPU treatment had no significant effect on the sucrose,fructose,and glucose content of mango fruit.However,256 mg/L concentration of CPPU treatment significantly reduced the fructose and sucrose content,and significantly increased the starch content.The two treatments of CPPU significantly increased the activity of mango AGP,and simultaneously reduced the activities of both SPS and AI,in particular,the 256 mg/L concentration of CPPU treatment reached a significant level.The correlation analysis results of fruit sugar component and enzyme activity showed that a positive correlation between starch content and AGP activity,the content of sucrose and fructose was significantly positively correlated with SPS activity,and the sucrose content was extremely significantly positively correlated with the fructose content.The mechanism by which high-quality concentration of 256 mg/L CPPU treatment reduced fruit sugar content might be through reducing SPS activity and sucrose content,while increasing AGP activity and fruit starch content,thereby delaying the conversion process of fruit starch to sugar.

关 键 词：台农1号芒果 CPPU 淀粉 糖分代谢 酶活性 

分 类 号：S667.7[农业科学—果树学]