构建基于体表神经源性渗出反应的急性胃黏膜损伤大鼠病情分级模型研究  

Establishment of a grading model for acute gastric mucosal injury in rats based on cutaneous neurogenic exudation response

作　　者：林栋[1] 齐诗仪 林锦文 王世豪 林丽莉[1,2] LIN Dong;QI Shiyi;LIN Jinwen;WANG Shihao;LIN Lili(College of Acupuncture and Massage,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Institute of Acupuncture and Meridian,Fujian Academy of Chinese Medical Sciences,Fuzhou 350003,China)

机构地区：[1]福建中医药大学针灸推拿学院,福州350122 [2]福建省中医药科学院经络研究所,福州350003

出　　处：《中国实验动物学报》2024年第8期976-984,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金面上项目(82074521);2024年度福建中医药大学针灸学科开放课题(XZJ2024001)。

摘　　要：目的构建不同浓度梯度盐酸诱导下的急性胃黏膜损伤(acute gastric mucosal injury,AGMI)大鼠模型,并探讨尾静脉注射不同浓度及剂量的伊文思蓝(evans blue,EB)对大鼠生存率及体表渗出情况的影响。方法(1)将Wistar大鼠根据体重随机分配到5个组:150~180 g组、180~200 g组、200~250 g组、300~400 g组以及400~500 g组,并进一步根据盐酸浓度分为8个亚组,具体浓度为:0.40、0.45、0.50、0.55、0.60、0.65、0.70 mol/L盐酸组以及以生理盐水组作为对照,共40个小组,每小组3只,共计120只。评估各组大鼠造模后24 h生存率,并分析体重和盐酸浓度对大鼠生存情况的交互关系。在确定5个梯度盐酸浓度的基础上,采用苏木精-伊红(HE)染色法观察胃黏膜镜下的病理变化。(2)选择(1)结果中适合制备的最高盐酸浓度制备AGMI模型,随后,将大鼠随机分配到不同的EB浓度和剂量组中,具体分组:EB 1(0.5%,0.4 mL)组、EB 2(1%,0.1 mL/100 g)组、EB 3(1%,0.2 mL/100 g)组、EB 4(2%,0.1 mL/100 g)组、EB 5(2%,0.2 mL/100 g)组、EB 6(5%,0.1 mL)组,每组5只,共30只。评估注射EB后24 h内的生存率和体表渗出程度。结果(1)AGMI大鼠造模后症状随着盐酸浓度升高而加剧,0.65 mol/L和0.70 mol/L在各体重组中的24 h生存率均为0%。Kaplan-Meier生存曲线表明,不同盐酸浓度组大鼠的生存率存在显著性差异(P<0.001),且盐酸浓度与体重之间的交互作用对大鼠的存活时间产生显著影响(P<0.001),确定的5个梯度盐酸浓度为0.40、0.45、0.50、0.55、0.60 mol/L。组织学观察结果表明,AGMI大鼠胃黏膜炎性细胞浸润程度随盐酸浓度升高而加剧。(2)0.60 mol/L盐酸制备的AGMI大鼠,尾静脉注射5%EB(0.1 mL)后24 h生存率仅为40%。Kaplan-Meier生存曲线结果显示,EB不同浓度和注射量下AGMI大鼠的生存率无显著性差异(P>0.05)。体表渗出情况分析显示,EB 1组大鼠皮肤和眼睛颜色变化较淡,体表渗出点较少,而EB 4组、EB 5组大鼠皮肤�Objective The aim of this study was to establish an acute gastric mucosal injury(AGMI)rat model induced by hydrochloric acid(HCl)at different concentration gradients and to investigate the effects of intravenous injection of evans blue(EB)at various concentrations and dosages on survival rate and superficial extravasation.Methods(1)Wistar rats were randomly assigned to five weight-based groups:150~180 g,180~200 g,200~250 g,300~400 g,400~500 g.Each group was further subdivided into eight subgroups based on HCl concentration,specifically:0.40,0.45,0.50,0.55,0.60,0.65,0.70 mol/L HCl,along with a control group treated with saline.This result ed in a total of 40 subgroups,with three rats per subgroup,summing up to 120 animals in total.The survival rate of rats 24 hours post-modeling was assessed,and the interaction between body weight and HCl concentration on rat survival was analyzed.Following the establishment of five graded HCl concentrations,gastric mucosal pathological changes were observed microscopically using hematoxylin-eosin staining(HE)staining.(2)From these result,the highest suitable concentration of HCl was selected to prepare the AGMI model.Subsequently,rats were randomly allocated into different groups based on the concentration and dose of EB,specifically:EB 1(0.5%,0.4 mL),EB 2(1%,0.1 mL/100 g),EB 3(1%,0.2 mL/100 g),EB 4(2%,0.1 mL/100 g),EB 5(2%,0.2 mL/100 g),and EB 6(5%,0.1 mL).Each group consisted of five rats,totaling 30 animals.The survival rate and extent of dermal exudation were evaluated 24 hours post-injection of EB.Results(1)Post-modeling symptoms in AGMI rats intensified with increasing concentrations of HCl,with the 24-hour survival rate in all weight groups being 0%for both 0.65 mol/L and 0.70 mol/L HCl.Kaplan-Meier survival curves indicated significant differences in survival rates among rats in different HCl concentration groups(P<0.001).Furthermore,a significant interaction effect between HCl concentration and body weight on rat survival time was observed(P<0.001).The five gradient HCl

关 键 词：穴位敏化 急性胃黏膜损伤 伊文思蓝 分布特征 

分 类 号：Q95-33[生物学—动物学]