醛固酮诱导多器官损害小鼠模型的研究  

Study on a mouse model of aldosterone-induced multi-organ damage

作　　者：罗玉 张海涛 郑亚威 孟宪泽 方震 王雅婷 方祝元[1] LUO Yu;ZHANG Haitao;ZHENG Yawei;MENG Xianze;FANG Zhen;WANG Yating;FANG Zhuyuan(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,南京210029

出　　处：《中国实验动物学报》2024年第8期1045-1051,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：江苏省研究生科研与实践创新计划项目(KYCX23_2139);江苏省中医高血压临床医学创新中心(k2021j17-1)。

摘　　要：目的建立与评价醛固酮诱导多脏器损害小鼠模型。方法小鼠20只随机分为4组,每组5只,分别为空白对照组(0μg/(kg·d))、醛固酮低剂量组(150μg/(kg·d)),醛固酮中剂量组(300μg/(kg·d)),醛固酮高剂量组(450μg/(kg·d)),通过手术在皮下埋置含有醛固酮的渗透性微泵,输注醛固酮4周建立醛固酮损害模型。每周记录小鼠体重、血压。4周造模结束后,小鼠处死取材,观察并分析小鼠血压及各脏器组织学形态等。结果(1)输注醛固酮4周后,醛固酮中、高剂量组小鼠血清中的醛固酮水平明显升高,而醛固酮低剂量组无明显升高;(2)小鼠置入渗透泵后,第2周与第3周醛固酮低、中、高剂量组收缩压均显著升高,但在第4周,醛固酮低、中、高剂量组血压均有所下降;(3)醛固酮低、中、高剂量组肾以及心脏均出现不同程度的损伤、细胞间质水肿、胶原沉积和纤维化病变;醛固酮低剂量组肝出现了少量的胶原沉积;醛固酮中、高剂量组有不同程度的的肝细胞损伤、胶原沉积和纤维化病变。结论醛固酮可以诱导小鼠的多脏器损害,在该种造模方式下脏器的损伤主要表现水肿、胶原沉积和纤维化病变。Objective Establishment and evaluation of a mouse model of aldosterone-induced multi-organ damage.Methods Twenty mice were randomly divided into four groups,with five mice in each group:a blank control group(0μg/(kg·d)),a low-dose aldosterone group(150μg/(kg·d)),a medium-dose aldosterone group(300μg/(kg·d)),and a high-dose aldosterone group(450μg/(kg·d)).Aldosterone-containing osmotic minipumps were surgically implanted under the skin,and aldosterone was infused for 4 weeks to establish the aldosterone-induced damage model.The body weight and blood pressure of the mice were recorded weekly.After the 4 week modeling period,the mice were euthanized,and their tissues were collected for observation and analysis of blood pressure and histological morphology of various organs.Results(1)After 4 weeks of aldosterone infusion,the serum aldosterone levels were significantly increased in the medium-dose and high-dose aldosterone groups,but not in the low-dose aldosterone group.(2)After the implantation of osmotic minipumps,the systolic blood pressure was significantly increased in the low-dose,medium-dose,and high-dose aldosterone groups during the second and third weeks,but decreased in all these groups during the fourth week.(3)The kidney and heart in the low-dose,medium-dose,and high-dose aldosterone groups showed varying degrees of damage,interstitial edema,collagen deposition,and fibrotic lesions.The liver in the low-dose aldosterone group showed a small amount of collagen deposition,while the medium-dose and high-dose aldosterone groups showed varying degrees of hepatocyte damage,collagen deposition,and fibrotic lesions.Conclusions Aldosterone can induce multi-organ damage in mice.Under this modeling method,organ damage is mainly manifested as edema,collagen deposition,and fibrotic lesions.

关 键 词：醛固酮 多脏器损伤 肾损伤 心脏损伤 肝损伤 小鼠 

分 类 号：Q95-33[生物学—动物学]