RP-HPLC-ELSD法测定逍遥颗粒中柴胡皂苷c、a、d的含量  

The Determination of Contents of Saikosaponins c,a,and d in Xiaoyao Granules by RP-HPLC-ELSD

作　　者：范洋 李明辉 逯小萌 王丽霞 FAN Yang;LI Ming-hui;LU Xiao-meng;WANG Li-xia(Puyang Food and Drug Inspection and Testing Center,Henan Province)

机构地区：[1]河南省濮阳市食品药品检验检测中心

出　　处：《中国食品药品监管》2024年第8期138-145,共8页China Food & Drug Administration Magazine

摘　　要：目的:采用反相高效液相色谱-蒸发光散射检测(RP-HPLC-ELSD)法同时测定逍遥颗粒中柴胡皂苷c、a、d三种成分的含量。方法:采用安捷伦(Agilent)SB-C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈(A)-水(B)。梯度洗脱程序为0~18min,30%A;18~26min,35%~45%A;26~35min,45%~55%A。流速为1.0ml/min,柱温为30℃,气体流速为2.5L/min,蒸发温度为60℃,进样量为10μl。结果:柴胡皂苷c、a、d分别在0.950~19.000μg、1.088~21.760μg、1.520~30.400μg的范围内线性关系良好。柴胡皂苷c平均回收率为99.49%,RSD为0.35%(n=9);柴胡皂苷a平均回收率为99.99%,RSD为0.66%(n=9);柴胡皂苷d平均回收率为99.12%,RSD为0.47%(n=9)。结论:该方法准确、灵敏、重复性好,可作为逍遥颗粒中柴胡皂苷c、a、d的含量测定方法。Objective:This study aims to determine the contents of saikosaponins c,a,and d in Xiaoyao granules using the RP-HPLC-ELSD method.Methods:An agilent SB-C18,chromatographic column(4.6mm×250mm,5μm)was utilized.The mobile phase consisted of acetonitrile(A)and water(B).The gradient elution procedure was as follows:0~18min,30%A;18~26min,35%~45%A;26~35min,45%~55%A.The flow rate was 1.0ml/min,the column temperature was 30°C,the gas flow rate was 2.5L/min,the evaporation temperature was 60°C,and the injection volume was 10μl.Results:Saikosaponins c,a,and d exhibited good linear relationships within the ranges of 0.9500~19.0000μg,0.1088~21.7600μg,and 1.5200~30.4000μg,respectively.The average recovery of saikosaponin c was 99.49%with an RSD of 0.35%(n=9);saikosaponin a had an average recovery of 99.99%with an RSD of 0.66%(n=9),and saikosaponin d had an average recovery of 99.12%with an RSD of 0.47%(n=9).Conclusion:The method is accurate,sensitive,and has good reproducibility.It is suitable for the determination of contents of saikosaponins c,a,and d in Xiaoyao granules.

关 键 词：逍遥颗粒 柴胡皂苷 反相高效液相色谱-蒸发光散射检测法 含量测定 质量控制 

分 类 号：R284.1[医药卫生—中药学]