加味银翘马勃散对小鼠放射性口腔黏膜炎的影响  

Effect of Modified Yinqiao Mabo Powder on Radiation Oral Mucositis in Mice

作　　者：杨琴 黄荣楷 蒋林亨 李桦康 林冰[3] Yang Qin;Huang Rongkai;Jiang Linheng;Li Huakang;Lin Bing(Chengdu University of Traditional Chinese Medicine,Chengdu,610075,China;The First Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou,510499,China;Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,610072,China)

机构地区：[1]成都中医药大学,四川成都610075 [2]广州中医药大学,广东广州510499 [3]成都中医药大学附属医院,四川成都610072

出　　处：《成都中医药大学学报》2024年第5期52-59,共8页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技计划项目(2020YFS0380)。

摘　　要：目的:探讨加味银翘马勃散对放射性口腔黏膜炎(Radiation-induced Oral Mucositis,RIOM)小鼠的预防治疗作用,并初步探讨该药物可能的作用机制。方法:将40只6-8周龄C57小鼠随机分为空白组、对照组、模型组、治疗组,每组10只。对小鼠头颈部以8 Gy剂量同一时间连续辐照3 d,建立RIOM小鼠模型。空白组采用不辐照并予以生理盐水灌胃,对照组采用不辐照并予以中药灌胃,模型组采用辐照并予以生理盐水灌胃,治疗组采用辐照并予以中药灌胃。自放疗前3 d开始灌胃进行预防性给药,直至取样日。监测体重,根据甲苯胺蓝染液、H&E染色评估RIOM严重程度;采用免疫组化、免疫荧光技术检测细胞增殖、凋亡;采用网络药理学分析加味银翘马勃散与疾病相关的信号通路,并检测相关蛋白表达。结果:与空白组相比,模型组小鼠总体质量下降(P<0.01);黏膜炎及溃疡面积占全舌面积升高(P<0.01);舌上皮细胞厚度和基底层细胞数量均减少(P<0.01);舌增殖标志蛋白PCNA表达降低(P<0.01);舌凋亡细胞表达水平升高(P<0.01);与疾病相关的PI3K、AKT蛋白表达水平升高(P<0.01)。与模型组相比,治疗组小鼠以上指标均改善(P<0.01)。结论:加味银翘马勃散的预防性治疗有利于RIOM的愈合,并可能通过下调PI3K和AKT蛋白的表达水平来抑制放射性口腔黏膜炎症。Objective:To investigate the preventive and therapeutic effect of Modified Yinqiao Mabo powder on radiation-induced oral mucositis(RIOM) mice,and the possible mechanism of the drug.Methods:Forty C57 mice aged 6-8 weeks were randomly divided into blank group,control group,model group and treatment group,with 10 mice in each group.To establish the RIOM mouse model,the head and neck of mice were irradiated with 8 Gy for 3 consecutive days.The control group was treated with non-irradiation and intragastric administration of Chinese medicine.The model group was treated with irradiation and intragastric administration of saline.Prophylactic administration was given by gavage from 3 days before radiotherapy until the day of sampling.Body weight was monitored,and the severity of RIOM was evaluated according to toluidine blue staining solution and H&E staining.Cell proliferation and apoptosis were detected by immunohistochemistry and immunofluorescence.Network pharmacology was used to analyze the signaling pathways related to Yinqiao Attenuation particles and detect the expression of related proteins.Results:Compared with the blank group,the total body weight of the model group was decreased(P<0.01).The area of mucositis and ulcer in the total tongue area increased(P<0.01).The thickness of tongue epithelial cells and the number of basal cells were decreased(P<0.01).The expression of tongue proliferation marker protein PCNA was decreased(P<0.01).The expression of apoptotic cells in tongue was increased(P<0.01).The protein expressions of PI3K and AKT related to the disease were increased(P<0.01).Compared with the model group,the above indicators of the treatment group were improved(P<0.01).Conclusion:The preventive treatment of Modified Yinqiao Mabo powder is beneficial to the healing of RIOM,such as reducing ulcer size,increasing epithelial cell thickness and basal layer epithelial cell composition,increasing epithelial cell proliferation and reducing cell apoptosis.Modified Yinqiao Mabo powder may inhibit the radioactive

关 键 词：辐照 放射性口腔黏膜炎 小鼠 网络药理学 

分 类 号：R289[医药卫生—方剂学] R242[医药卫生—中药学]