TCM-HIN2Vec:A strategy for uncovering biological basis of heart qi deficiency pattern based on network embedding and transcriptomic experiment  

作　　者：Lihong Diao Xinyi Fan Jiang Yu Kai Huang Edouard C.Nice Chao Liu Dong Li Shuzhen Guo 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China [2]Department of Biochemistry and Molecular Biology,Monash University,Clayton VIC 3800,Australia [3]Beijing Institute of Lifeomics,Beijing 102206,China [4]State Key Laboratory of Medical Proteomics,Beijing 102206,China [5]National Center for Protein Sciences(Beijing),Beijing 102206,China [6]Beijing Proteome Research Center,Beijing 102206,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2024年第3期264-274,共11页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(32088101);National key Research and Development Program of China(2017YFC1700105,2021YFA1301603).

摘　　要：Objective:To elucidate the biological basis of the heart qi deficiency(HQD)pattern,an in-depth understanding of which is essential for improving clinical herbal therapy.Methods: We predicted and characterized HQD pattern genes using the new strategy,TCM-HIN2Vec,which involves heterogeneous network embedding and transcriptomic experiments.First,a heterogeneous network of traditional Chinese medicine(TCM)patterns was constructed using public databases.Next,we predicted HQD pattern genes using a heterogeneous network-embedding algorithm.We then analyzed the functional characteristics of HQD pattern genes using gene enrichment analysis and examined gene expression levels using RNA-seq.Finally,we identified TCM herbs that demonstrated enriched interactions with HQD pattern genes via herbal enrichment analysis.Results: Our TCM-HIN2Vec strategy revealed that candidate genes associated with HQD pattern were significantly enriched in energy metabolism,signal transduction pathways,and immune processes.Moreover,we found that these candidate genes were significantly differentially expressed in the transcriptional profile of mice model with heart failure with a qi deficiency pattern.Furthermore,herbal enrichment analysis identified TCM herbs that demonstrated enriched interactions with the top 10 candidate genes and could potentially serve as drug candidates for treating HQD.Conclusion: Our results suggested that TCM-HIN2Vec is capable of not only accurately identifying HQD pattern genes,but also deciphering the basis of HQD pattern.Furthermore our finding indicated that TCM-HIN2Vec may be further expanded to develop other patterns,leading to a new approach aimed at elucidating general TCM patterns and developing precision medicine.

关 键 词：Qi deficiency pattern Heart failure Biological basis Network embedding Transcriptome 

分 类 号：R259[医药卫生—中西医结合]