Mechanism of Guangdong Shenqu in regulating intestinal flora in mice with food stagnation and internal heat based on 16S rDNA sequencing  

作　　者：Yani Jiang Zhenhong Zhang Han Chen Yanmin Wang Yanrui Xu Zijie Chen Yan Xu Jingjuan Wang 

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing,102488,China [2]School of Chinese Traditional Medicine,Beijing University of Chinese Medicine,Beijing,102488,China [3]Department of Chemistry,Cleveland State University,Cleveland,44115,USA

出　　处：《Journal of Traditional Chinese Medical Sciences》2024年第2期232-244,共13页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(81872995).

摘　　要：Objective:To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA sequencing.Methods: Mice were randomly assigned to control,model,GSQ low-dose(GSQL),GSQ medium-dose(GSQM),GSQ high-dose(GSQH),and lacidophilin tablets(LAB)groups,with each group containing 10 mice.A food stagnation and internal heat mouse model was established through intragastric administration of a mixture of beeswax and olive oil(1:15).The control group was administered normal saline,and the model group was administered beeswax and olive oil to maintain a state.The GSQL(2 g/kg),GSQM(4 g/kg),GSQH(8 g/kg),and LAB groups(0.625 g/kg)were administered corresponding drugs for 5 d.After administration,16S rDNA sequencing was performed to assess gut microbiota in mouse fecal samples.Results: The model group exhibited significant intestinal flora changes.Following GSQ administration,the abundance and diversity index of the intestinal flora increased significantly,the number of bacterial species was regulated,andαandβdiversity were improved.GSQ administration increased the abundance of probiotics,including Clostridia,Lachnospirales,and Lactobacillus,whereas the abundance of conditional pathogenic bacteria,such as Allobaculum,Erysipelotrichaceae,and Bacteroides decreased.Functional prediction analysis indicated that the pathogenesis of food stagnation and GSQ intervention were primarily associated with carbohydrate,lipid,and amino acid metabolism,among other metabolic pathways.Conclusion: The digestive mechanism of GSQ may be attributed to its role in restoring diversity and abundance within the intestinal flora,thereby improving the composition and structure of the intestinal flora in mice and subsequently influencing the regulation of metabolic pathways.

关 键 词：Guangdong Shenqu Food stagnation Gut microbiota High-throughput gene sequencing Species annotation Metabolic pathway 

分 类 号：R285.5[医药卫生—中药学]