microRNA-2184 orchestrates Mauthner-cell axon regeneration in zebrafish via syt3 modulation  

作　　者：Xinghan Chen Yueru Shen Zheng Song Xinliang Wang Huaitong Yao Yuan Cai Zi-Ang Zhao Bing Hu 

机构地区：[1]Hefei National Research Center for Physical Sciences at the Microscale,University of Science and Technology of China,Hefei,Anhui 230026,China [2]The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230026,China [3]Center for Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230026,China

出　　处：《Journal of Genetics and Genomics》2024年第9期911-921,共11页遗传学报（英文版）

基　　金：supported by the Research Funds of the Center for Advanced Interdisciplinary Science and Biomedicine of IHM(QYZD20220002);the National Natural Science Foundation of China(82071357);the Ministry of Science and Technology of China(2019YFA0405600 to B.H.).

摘　　要：MicroRNAs(miRNAs)play a significant role in axon regeneration following spinal cord injury.However,the functions of numerous miRNAs in axon regeneration within the central nervous system(CNS)remain largely unexplored.Here,we elucidate the positive role of microRNA-2184(miR-2184)in axon regeneration within zebrafish Mauthner cells(M-cells).The upregulation of miR-2184 in a single M-cell can facilitate axon regeneration,while the specific sponge-induced silencing of miR-2184 leads to impeded regeneration.We show that syt3,a downstream target of miR-2184,negatively regulates axon regeneration,and the regeneration suppression modulated by syt3 depends on its binding to Ca^(2+).Furthermore,pharmacological stimulation of the cAMP/PKA pathway suggests that changes in the readily releasable pool may affect axon regeneration.Our data indicate that miR-2184 promotes axon regeneration of M-cells within the CNS by modulating the downstream target syt3,providing valuable insights into potential therapeutic strategies.

关 键 词：Axon regeneration Axon ablation miR-2184 syt3 Readily releasable pool 

分 类 号：Q95[生物学—动物学]