市售药食同源保健花茶物种鉴定研究  

Research on species identification of commercial medicinal and food homology scented herbal tea

作　　者：孙靖 黄子怡 李思齐 李余芳 胡艳 郭世文 胡格 申传璞 杨芙蓉 林余霖 辛天怡 浦香东 SUN Jing;HUANG Zi-yi;LI Si-qi;LI Yu-fang;HU Yan;GUO Shi-wen;HU Ge;SHEN Chuan-pu;YANG Fu-rong;LIN Yu-lin;XIN Tian-yi;PU Xiang-dong(School of Pharmacy,Anhui Medical University,Hefei 230032,China;Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the Peoples Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)

机构地区：[1]安徽医科大学药学院,安徽合肥230032 [2]中国医学科学院、北京协和医学院药用植物研究所,国家中医药管理局中药资源保护重点研究室,北京100193

出　　处：《药学学报》2024年第9期2612-2624,共13页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(82204346);安徽省高校优秀青年基金(2022AH030078);安徽医科大学校科研基金项目(2023xkj011);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-022)。

摘　　要：掺伪混伪严重影响药材质量,进而危及人类健康。药食同源保健食品属于中药衍生物,相比于药物而言,其更易获取且贴近消费者生活。然而,市售药食同源保健食品中的中药组分真伪鉴定尚未引起重视,且无明确标准可依,急需开展系统性研究。本研究基于DNA条形码分子鉴定技术,结合ITS2、psbA-trnH、matK通用条形码序列及DX、HH、JYH特异性条形码序列对市售药食两用保健花茶真伪进行鉴定,旨在对市场上流通的药食同源保健花茶产品真伪情况进行调查。研究结果显示:180份市售产品中共有164份样品成功获得DNA条形码序列,其余样品因无目标组分或DNA存在降解,无法获得扩增产物。结合性状鉴定研究发现180份样品中共有141份为正品,占总样品量的78.33%;31份样品存在掺伪现象,8份样品无目标组分,共占总样品量的21.67%。此外,水试研究发现,5份红花花茶样品存在红花掺重现象。本研究揭示药食同源保健花茶产品质量存在掺伪及掺重情况,提示监管部门应该加速药食同源保健食品领域质量标准的建立,为药食同源保健食品产业的快速发展提供参考。The adulteration and counterfeiting of herbal ingredients in medicinal and food homology(MFH)have a serious impact on the quality of herbal materials,thereby endangering human health.Compared to pharmaceutical drugs,health products derived from traditional Chinese medicine(TCM)are more easily accessible and closely integrated into consumers'daily life.However,the authentication of the authenticity of TCM ingredients in MFH has not received sufficient attention.The lack of clear standards emphasizes the necessity of conducting systematic research in this area.This study utilized DNA barcoding technology,combining ITS2,psbA-trnH,matK as universal barcode sequences,and DX,HH,JYH as specific barcode sequences,to identify the authenticity of commercial medicinal and food homology scented herbal teas.The aim was to investigate the authenticity of scented herbal tea products circulating in the market.The research results revealed that among 180 scented herbal tea samples,DNA barcodes were successfully obtained from 164 samples,while the remaining samples either lacked the target components or suffered severe DNA degradation,resulting in failed amplification.Combined with morphological identification studies,it was found that out of the 180 samples,141 were authentic,accounting for 78.33%of the total samples,while 31 samples showed adulteration and 8 samples lacked the target components,accounting for 21.67%of the total samples.Additionally,water testing revealed that 5 samples of Carthamus tinctorius herbal tea exhibited the phenomenon of weight adulteration.This study exposed the presence of adulteration and weight adulteration in scented herbal tea products,highlighting the need for regulatory authorities to expedite the establishment of quality standards in scented herbal tea industry.These findings provide valuable insights for the rapid development of the scented herbal tea industry.

关 键 词：药食同源 中药花茶 分子鉴定 DNA条形码 真伪 

分 类 号：R931[医药卫生—生药学]