猪METTL3基因生物信息学分析及靶基因预测  

Bioinformatics analysis and target gene prediction of porcine METTL3 gene

作　　者：秦睿 王文强 韦梦姜 杨耀[2] 焦润 王心如 杨伟炳 张娟丽[1,3] QIN Rui;WANG Wenqiang;WEI Mengjiang;YANG Yao;JIAO Run;WANG Xinru;YANG Weibing;ZHANG Juanli(School of Life Science and Technology,Longdong University,Qingyang 745000;Gannan Tibetan Autonomous Prefecture Animal Disease Prevention and Control Center,Hezuo 737400;Gansu Key Laboratory of Conservation and Utilization of Biological Resources and Ecological Restoration,Qingyang 745000,China)

机构地区：[1]陇东学院生命科学与技术学院,甘肃庆阳745000 [2]甘南藏族自治州动物疫病预防控制中心,甘肃合作737400 [3]甘肃省陇东生物资源保护利用与生态修复重点实验室,甘肃庆阳745000

出　　处：《生物技术》2024年第4期431-438,共8页Biotechnology

基　　金：甘肃省教育科技创新项目(2024A-162);陇东学院创新创业启动基金项目(202301144);陇东学院博士科研启动基金项目(XYBYZK2210)。

摘　　要：[目的]获得猪METTL3蛋白理化性质、二、三级结构、蛋白保守性、甲基化位点及靶基因等信息,为进一步了解猪METTL3基因的功能提供了理论依据。[方法]利用生物信息学软件对猪METTL3蛋白质的理化性质、二、三级结构及保守结构域、甲基化位点、蛋白互作和靶基因等进行预测分析。[结果]猪METTL3为亲水性不稳定蛋白,主要由无规卷曲和α-螺旋、β-转角构成,主要存在于细胞核中,与METTL14、WTAP和POLR2B等蛋白具有互作关系。METTL3有4个高可信度m^(6)A位点,经M6A2 Target软件预测该基因与CASP1、PYCR1、RANBP2、LIRB4、APOBEC3A、ADAM19等134个基因存在靶向关系。[结论]猪METTL3是一种主要存在于细胞核中的亲水性不稳定蛋白,主要通过m^(6)A甲基化修饰作用于靶基因而发挥作用。[Objective] The physical and chemical properties, secondary and tertiary structure, protein conservation, methylation site and target gene of porcine METTL3 protein were obtained, which provided theoretical basis for further understanding the function of porcine METTL3 gene.[Method] The physical and chemical properties, secondary and tertiary structures, conserved domains, methylation sites, protein interactions and target genes of porcine METTL3 protein were predicted by bioinformatics software.[Result] Porine METTL3 is a hydrophilic unstable protein, mainly composed of random curling, α-helix and β-corner, which mainly exists in the nucleus and has interaction with METTL14,WTAP and POLR2B proteins.METTL3 has four high-confidence m^(6)A sites, and M6A2 Target software predicted that this gene has a targeting relationship with 134 genes, including CASP1,PYCR1,RANBP2,LIRB4,APOBEC3A and ADAM19.[Conclusion] Porcine METTL3 is a hydrophilic unstable protein that exists mainly in the nucleus and exerts its effects on target genes mainly through m^(6)A methylation modification.

关 键 词：METTL3基因 蛋白理化性质 保守性 亚细胞定位 甲基化位点 MOTIF 靶基因预测 

分 类 号：S828[农业科学—畜牧学]