金银花根腐病病原菌分离鉴定及其生物学特性分析  

Isolation and identification of the pathogen of honeysuckle root rot and analysis of its biological characteristics

作　　者：周英森 章淑艳 贾振华 李冉[3] 姜茗轩 王玥 韩韬 王茂存 刘紫烟 纪佳星 ZHOU Yingsen;ZHANG Shuyan;JIA Zhenhua;LI Ran;JIANG Mingxuan;WANG Yue;HAN Tao;WANG Maocun;LIU Ziyan;JI Jiaxing(Hebei Academy of Sciences,Shijiazhuang 050052;Hebei Institute of Microbiology Co.,Ltd.,Baoding 071051;Institute of Biology,Hebei Academy of Sciences,Shijiazhuang 050052;Julu Institute of Applied Technology,Xingtai 055250,China)

机构地区：[1]河北省科学院,河北石家庄050052 [2]河北省微生物研究所有限公司,河北保定071051 [3]河北省科学院生物研究所,河北石家庄050052 [4]巨鹿应用技术研究院,河北邢台055250

出　　处：《生物技术》2024年第4期454-460,447,共8页Biotechnology

基　　金：河北省科学院基本科研业务费制度试点项目(2022PF02-3);河北省科学院高层次人才培养与资助项目(2023G240);河北省现代农业产业技术体系专项资金(HBCT2024110207)。

摘　　要：[目的]探明引起河北巨鹿县的金银花田部分植株出现叶片变褐干枯,根部变褐,茎基表面附有白色菌丝等现象的病原。[方法]组织分离法分离病部菌株,用科赫法则验证其致病性,通过形态学及分子生物学鉴定菌株物种,并研究了该菌株的生物学特性。[结果]分离到的病原菌株B22与Fusarium equiseti同源性达99%以上,确定为木贼镰孢菌(Fusarium equiseti)。生物学特性研究结果:OMA培养基最适其菌丝生长,最适温度为28℃,最适pH值为7,光照对其影响较小,致死温度为61℃。[结论]从金银花病害中分离鉴定出一病原菌,即木贼镰孢菌;分析了其生物学特性,该菌株在pH值7的OMA培养基质中,28℃下生长最快,光照对其影响较小,而在温度61℃中10 min致死;可为金银花同种病害的诊断及防控提供理论根据。Hebei Academy of Sciences;Julu Institute of Applied Technology;Hebei Institute of Microbiology Co.,Ltd.;Institute of Biology,Hebei Academy of Sciences;[Objective]In order to clarify the causes of the phenomena such as leaf loss,yellowing,browning and drying,root browning,and attachment of white mycelium on the surface of the stem base,some plants in honeysuckle fields in Julu County,Hebei Province,China,in recent years.[Method]The diseased strain was isolated by tissue isolation method,its pathogenicity was verified using Koch′s law,and the strain species was identified by morphology and ITS sequences,followed by biological characterization of the strain.[Result]A pathogenic strain B22 was isolated,and the homology with Fusarium equiseti was more than 99%,which was identified as Fusarium equiseti.The results of biological characterization showed that the OMA medium was the most suitable for the growth of its mycelium,the optimal growth temperature was 28℃,the optimal pH value was 7,the effect of light on it was small,and the lethal temperature was 61℃.[Conclusion]The pathogenic bacterium,namely Fusarium equiseti,was isolated and identified from the honeysuckle disease,and its biological characteristics were analyzed,This strain grew fastest at 28℃in OMA culture medium at pH 7,and was less affected by light,but was lethal at 61℃for 10 min.This study can provide a relatively objective basic theoretical basis for the diagnosis,prevention and control of honeysuckle homogeneous diseases.

关 键 词：金银花 病原菌 木贼镰孢菌 科赫法则 组织分离法 致病性 生物学特性 

分 类 号：S432.4[农业科学—植物病理学]