短期使用污泥产品对汞在土壤–国槐树体迁移和积累的影响  

Effects of short-term application of sludge products on migration and accumulation of mercury in soil-Styphnolobium japonicum system

作　　者：陈玲[1] 朱坤 彭祚登[1] 熊建军[2] 李淑文 Chen Ling;Zhu Kun;Peng Zuodeng;Xiong Jianjun;Li Shuwen(Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,China;Beijing City Drainage Refco Group Ltd,Beijing 100044,China;Daxing District Fruit and Forestry Research Institute,Beijing 100162,China)

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]北京城市排水集团有限责任公司,北京100044 [3]北京市大兴区果林研究所,北京100162

出　　处：《北京林业大学学报》2024年第9期87-96,共10页Journal of Beijing Forestry University

基　　金：国家发改委环境污染第三方治理项目(2017HXFWLXY023)。

摘　　要：【目的】研究城镇生活污泥产品中重金属汞(Hg)在土壤–国槐树体中的迁移和积累,探讨国槐幼苗对Hg胁迫的响应机制,明确污泥产品在国槐幼苗抚育方面的应用潜力。【方法】以国槐幼苗为研究对象,通过盆栽试验,设置3个污泥使用量梯度(0、2、4 kg/m^(2))和4个取样时间(40、80、120、160 d),将处理分为移栽国槐组和纯土壤组,探究土壤Hg含量的动态变化,进一步研究Hg在国槐根系和叶片的富集迁移规律、亚细胞分布特点及赋存形态。【结果】(1)随污泥产品使用时间增加,土壤Hg含量显著降低,在种植国槐植株土壤中添加污泥产品,短期内土壤Hg含量显著低于纯土壤。(2)污泥产品用量、取样时间及其交互作用均对国槐根和叶中的Hg含量有显著影响。污泥施用40和160 d国槐根系和叶片对Hg的吸收显著高于未施污泥组,使用污泥产品前期会促进Hg在根、叶间的转移,后期抑制。污泥产品的低施用量可以显著增加国槐生物量从而提高对Hg的积累量。(3)在国槐根、叶中,Hg主要分布在细胞壁和可溶性组分中,两者总占比超过90%。(4)国槐根系和叶片中Hg的残渣态占比最大,分别占比57.2%~59.7%和52.6%~69.0%。【结论】国槐可以应对低施用量污泥产品带来的Hg胁迫,主要途径为细胞壁固定、液泡区隔化以及将Hg转化为低活性形态贮存。[Objective]This paper aims to investigate the migration and accumulation of heavy metal mercury(Hg)in the soil-Styphnolobium japonicum system from sludge products in urban life,so as to explore the response mechanism of S.japonicum seedlings to Hg stress,and to clarify the application potential of sludge products in cultivation of S.japonicum seedlings.[Method]The study focused on S.japonicum seedlings,employing a pot experiment to establish three gradients of sludge application rates(0,2,and 4 kg/m^(2))and four sampling times(40,80,120,and 160 d).The treatments were divided into a transplanted S.japonicum group and a pure soil group to explore the dynamic changes in soil Hg content.The study further investigated the enrichment and migration patterns of Hg in the roots and leaves of S.japonicum,as well as the characteristics of subcellular distribution and the forms of existence.[Result](1)With the increase in the duration of sludge product application,the soil Hg content significantly decreased.In the soil planted with S.japonicum,the addition of sludge products resulted in significantly lower Hg content compared with pure soil in short term.(2)The amount of sludge product,sampling time,and their interaction all significantly affected the Hg content in the roots and leaves of S.japonicum.At 40 and 160 d,the application of sludge products significantly promoted the absorption of Hg by roots and leaves of S.japonicum.The use of sludge products in early stage can promote the transfer of Hg between roots and leaves,while in the later stage,it inhibited it.A low application rate of sludge products can significantly increase the biomass of S.japonicum,thereby increasing the accumulation of Hg.(3)In the roots and leaves of S.japonicum,Hg was mainly distributed in cell wall and soluble fractions,with a combined proportion exceeding 90%.(4)The residual form of Hg in roots and leaves of S.japonicum accounted for the largest proportion,with a range of 57.2%to 59.7%and 52.6%to 69.0%,respectively.[Conclusion]S.japonicum can

关 键 词：污泥 国槐 汞 亚细胞分布 化学形态 

分 类 号：S792.26[农业科学—林木遗传育种]