受枯梢病侵染的有症状和无症状樟子松的内生真菌群落结构  

Community structure of endophytic fungi in asymptomatic and symptomatic Pinus sylvestris var.mongolica infected by diplodia tip blight

作　　者：刘玉祥 王磊 任宁红 游崇娟[1] Liu Yuxiang;Wang Lei;Ren Ninghong;You Chongjuan(Beijing Key Laboratory of Forest Pest Control,Beijing Forestry University,Beijing 100083,China;Forestry Protection Station,Honghuaerji Forestry Bureau,Hulunbuir 165456,Inner Mongolia,China)

机构地区：[1]北京林业大学林木有害生物防治北京市重点实验室,北京100083 [2]内蒙古呼伦贝尔市红花尔基林业局森防站,内蒙古呼伦贝尔165456

出　　处：《北京林业大学学报》2024年第9期119-131,共13页Journal of Beijing Forestry University

基　　金：国家重点研发计划项目(2021YFC2600400);政府间国际科技创新合作(2023YFE0116200)。

摘　　要：【目的】分析受樟子松枯梢病侵染的无症状和有症状樟子松不同部位的内生真菌多样性和群落结构,以期为筛选有效的内生拮抗菌并防治樟子松枯梢病提供科学依据。【方法】利用分离培养和高通量测序技术,对无症状和有症状樟子松的针叶、嫩梢、韧皮部(胸径处)3个部位内生真菌群落的多样性和结构进行了比较和分析,并筛选了嫩梢处丰度有显著差异的内生菌。【结果】(1)分别在无症状和有症状樟子松中分离到233株和360株内生真菌,分离率最高的均是Diplodia sapinea。此外,Hormonema sp.在无症状樟子松上分离率较高,但在有症状樟子松上分离率较低,且仅在有症状樟子松的嫩梢处分离到。(2)扩增子测序结果显示:无症状和有症状樟子松中内生真菌的α多样性无显著差异,但无症状和有症状樟子松的不同部位内生真菌群落结构存在差异。(3)对扩增子数据进行线性判别分析发现:Hormonema属真菌在无症状樟子松嫩梢中丰度最高,而Diplodia属真菌在有症状樟子松的嫩梢中丰度最高。(4)真菌营养型分类也证实无症状樟子松的3个部位中均是腐生菌的相对丰度最高,而有症状樟子松的韧皮部的植物病原菌的相对丰度较无症状显著增多。【结论】无症状和有症状樟子松中内生真菌的多样性无显著差异,但其不同部位内生真菌群落结构存在显著差异。下一步将进行拮抗试验以评估无症状樟子松嫩梢部位中显著富集的真菌Hormonema属真菌作为生防菌的潜力。[Objective]Diplodia tip blight of Mongolian pine is widely distributed in Honghuaerji,Inner Mongolia of northern China,causing severe damage on natural Mongolian pine(Pinus sylvestris var.mongolica).Our study analyzed the diversity and community structure of endophytic fungi in different parts of asymptomatic and symptomatic Mongolian pine infected by Diplodia sapinea,laying a research foundation for the subsequent screening of effective endophytic antagonistic strains and the control of diplodia tip blight.[Method]Using culture-based and high-throughput sequencing techniques,the diversity and structure of endophytic fungal communities in different tissues(needles,twigs and phloem)of asymptomatic and symptomatic Mongolian pine were compared and analyzed,and endophytic fungi with significant differences in abundance at twigs were screened.[Result](1)360 and 233 taxa of endophytic fungi were isolated from symptomatic and asymptomatic Mongolian pine,respectively.D.sapinea was isolated from both asymptomatic and symptomatic Mongolian pine,with the highest isolation rate.In addition,Hormonema sp.had a higher isolation rate on asymptomatic Mongolian pine,but a lower isolation rate on symptomatic Mongolian pine,and it was only isolated from the diseased twigs.(2)The amplicon sequencing results showed that there were no significant differences ofαdiversity of endophytic fungi between symptomatic and asymptomatic Mongolian pine,but beta diversity,community composition and structure of endophytic fungi were various according to different tissues from symptomatic and asymptomatic Mongolian pine.(3)Linear discriminant analysis effect size(LEfSe)analysis of amplicon revealed that the fungus Diplodia sp.was significantly enriched in the twigs of symptomatic Mongolian pine,while Hormonema sp.was significantly enriched in the twigs of asymptomatic Mongolian pine.(4)Fungal trophic classification confirmed that the relative abundance of saprophytic fungi was highest in all three parts of asymptomatic Mongolian pine,while the rela

关 键 词：病虫害防治 樟子松 枯梢病 真菌 群落结构 高通量测序 

分 类 号：S718.5[农业科学—林学]