Saponin Ⅰ from Shuitianqi(Rhizoma Schizocapasae Plantagineae) inhibits metastasis by negatively regulating the transforming growth factor-β1/Smad7 network and epithelial-mesenchymal transition in the intrahepatic metastasis Bagg's Albino/c mouse model  

作　　者：LYU Meixian ZHOU Huan ZHI Limin ZHOU Jinling GAN Rizhi QIN Yanping HE Nengting ZUO Qiqi LI Hao DONG Min LIANG Gang 

机构地区：[1]Pharmaceutical College,Guangxi Medical University,Nanning 530022,China [2]Department of Pharmacy,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530022,China [3]Drug clinical trial Facility office,the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530022,China [4]College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530022,China [5]Department of Pharmacy,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530005,China [6]Department of Pharmacy,People's Hospital of Guangxi Hezhou,Hezhou 542899,China [7]Department of Pharmacy,Guangzhou Women and Children's Medical Center Liuzhou Hospital,Liuzhou 545000,China [8]Department of Pharmacy,First Affiliated Hospital of Gannan Medical University,Gannan 341000,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第4期642-651,共10页中医杂志（英文版）

基　　金：National Natural Science Foundation of China,a New Anti-cancer Plant drug,SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae),against Invasion and Metastasis of Non-small Cell Lung Cancer and Reversing Tyrosine Kinase Inhibitors Resistance basing on Human Growth Factor/c-Mesenchymal to Epithelial Transition Factor Pathway and its Molecular Mechanism of Regulating Epithelial-Mesenchymal Transition(No.8164062);the Natural Science Foundation of Guangxi Province,Study on the Antihepatic Fibrosis Mechanism of Saponins from Shuitianqi(Rhizoma Schizocapasae Plantagineae)based on Transforming Growth Factor-β/Smad Signaling Pathway(No.2019GXNSFAA245075)。

摘　　要：OBJECTIVE:To examine the influence of SaponinⅠfrom Shuitianqi(Rhizoma Schizocapasae Plantagineae)(SSPHⅠ)on hepatocellular carcinoma(HCC)metastasis,and to elucidate the underlying mechanism.METHODS:The intrahepatic metastasis Bagg's Albino/c(BALB/c)mouse model was established with human hepatocellular carcinomas(HepG2)cells,then treated with normal saline(once per day),cisplatin(2 mg/kg,once every 2 d),and SSPHⅠ(25,50,and 75 mg/kg,once per day).Then,we assessed alterations in the hepatic pathology and target protein expressions in the intrahepatic metastasis BALB/c mouse model using a series of molecular biology techniques.RESULTS:Based on our analysis,SSPHⅠsignificantly alleviated hepatocyte necrosis and tumor cells infiltration.Moreover,SSPHⅠsuppressed extracellular matrix(ECM)degradation and angiogenesis via a decrease in matrix etalloproteinase-2(MMP-2),MMP-9,CD31,CD34,and vascular endothelial growth factor(VEGF)levels.Furthermore,SSPHⅠrepressed invasion and metastasis by suppressing the transforming growth factor-β1(TGF-β1)/Smad7 axis and epithelial-mesenchymal transition(EMT),as evidenced by the scarce TGF-β1,Ncadherin,and Vimentin expressions,and elevated Smad7 and E-cadherin expressions.CONCLUSION:The SSPHⅠ-mediated negative regulation of the TGF-β1/Smad7 axis and EMT are critical for the inhibition of HCC invasion and metastasis.

关 键 词：carcinoma hepatocellular epithelial-mesenchymal transition transforming growth factor beta1 Smad7 protein Saponin I from Shuitianqi(Rhizoma Schizocapasae Plantagineae) 

分 类 号：R285.5[医药卫生—中药学]