Study on the four Qi of Pfaffia glomerata based on the metabolomics technology and comparison of Dangshen(Radix Codonopsis) in the equivalent substitution prescription  

作　　者：SONG Zhenguang YANG Bin WANG Fei YAN Dongmei ZHOU Xiaoqing HUANG Liping GAO Xuemei LI Bin HUANG Luqi 

机构地区：[1]Academician Workstation,Jiangxi University of Chinese Medicine,Nanchang 330004,China [2]State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [3]National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences(CACMS),Beijing 100700,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第4期713-721,共9页中医杂志（英文版）

基　　金：2018 National Key Research and Development Project:Research on Four Modes of Exploitation of New Sources of Rare and Endangered Chinese Medicine Resources-Research on Foreign Medicinal Materials(No.2018YFC1706100);Jiangxi Provincial Natural Science Foundation Key Project:Synthesis and Properties of Polysaccharide Moleculally-Imprinted Core-shell Microspheres based on Magnetic Micelle Technology(No.20192ACBL20028);。

摘　　要：OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese medicine.METHODS:The normal group,six cold herb groups,six hot herb groups,six cool herb groups,PG prescription group(PGPG),and Dangshen(Radix Codonopsis)prescription group(CPPG)were prepared with corresponding concentrations of water extracts,these herb extracts were administered by gavage to SpragueDawley rats,and the 12 h urine at night on the 29th day of the SD rats in each group were collected,Liquid Chromatograph Mass Spectrometer system was used to analyze them,the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up,so as to the medicinal properties of PGPG,CPPG and PG were predicted.Based on the Progenesis QI,Human Metabolome Database,Kyoto Encyclopedia of Genes and Genomes,MetaboAnalyst 5.0 database,we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG prescription.RESULTS:We established a best model of cold-hot herbal discrimination in the positive ion mode,then the probability that PGPG was predicted cold property was 88.9%.Furthermore,a model of cold-cool herbal discrimination was established,then the probability of PGPG containing the cool property was 77.8%.In addition,typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline,(R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in rats.The regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine metabolism.CONCLUSION:The results showed PGPG had a cool medicinal property as same as CPPG,and the regulation trend of PGPG on markers was consistent with cool herbs.Therefore,the medicine properties PG and CP should be consistent,and the Traditional Chinese Medicine property of PG was predicted to be neutral.

关 键 词：Pfaffia glomerata medicinal properties of Chinese medicine metabolomics molecular mechanism equivalent substitution 

分 类 号：R285.5[医药卫生—中药学]