补中益气汤对A549细胞荷瘤裸鼠移植瘤MRP、LRP、P-gp表达的影响  

Effects of Buzhong Yiqi Decoction on the expressions of MRP,LRP and P-gp in transplantation tumor of A549 tumor-bearing nude mice

作　　者：刘玥彤 牟琪瑞 李贺[1] 黄婧漪 高原[1] Liu Yuetong;Mu Qirui;Li He;Huang Jingyi;Gao Yuan(Pathological Teaching and Research Office Affiliated to Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学病理教研室,沈阳110847

出　　处：《国际中医中药杂志》2024年第9期1145-1149,共5页International Journal of Traditional Chinese Medicine

基　　金：辽宁省教育厅科学研究面上项目(LJKZ0901);辽宁中医药大学中西医结合学院“岐济”人才支撑计划项目(2023QJ01001)。

摘　　要：目的探讨补中益气汤对A549细胞荷瘤裸鼠移植瘤中多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)、P-糖蛋白(P-gp)表达的影响。方法培养A549细胞建立荷瘤裸鼠移植瘤模型,按随机数字表法分为空白对照组、顺铂组及顺铂联合补中益气汤低、中、高剂量组。相应药物干预25 d后,采用RT-qPCR测定移植瘤MRP、LRP、P-gp mRNA水平,Western Blot检测移植瘤组织MRP、LRP、P-gp蛋白表达情况。结果与空白对照组比较,顺铂组MRP、LRP、P-gp mRNA及蛋白表达降低(P<0.05);与顺铂组比较,顺铂联合补中益气汤低、中、高剂量组MRP、LRP、P-gp mRNA水平及蛋白表达降低(P<0.05)。结论补中益气汤可抑制A549细胞荷瘤裸鼠移植瘤MRP、LRP、P-gp表达,且无剂量依赖性。Objective To investigate the effects of Buzhong Yiqi Decoction on the expressions of multidrug resistance-related protein(MRP),lung resistance-related protein(LRP),and P-glycoprotein(P-gp)in transplantation tumor of A549 tumor-bearing nude mice.Methods A549 cells were cultured to establish xenograft models of tumor-bearing nude mice.According to the random number table method,they were divided into blank control group,cisplatin group,cisplatin+Buzhong Yiqi Decoction low-dosage group,cisplatin+Buzhong Yiqi Decoction medium-dosage group,cisplatin+Buzhong Yiqi Decoction high-dosage group.After 25 days of corresponding drug intervention,the mRNA expressions of MRP,LRP and P-gp in transplantation tumors were detected by Real-Time PCR,and Western blot was used to detect the expressions of MRP,LRP and P-gp proteins in xenograft tissues in each group.Results Compared with blank control group,the mRNA and protein expressions of MRP,LRP and P-gp in cisplatin group were significantly down-regulated(P<0.05).Compared with cisplatin group,the mRNA and protein expressions of MRP,LRP and P-gp in the cisplatin+Buzhong Yiqi Decoction low-dosage group,cisplatin+Buzhong Yiqi Decoction medium-dosage group,cisplatin+Buzhong Yiqi Decoction high-dosage group decreased(P<0.05).Conclusion Buzhong Yiqi Decoction can inhibit the expressions of MRP,LRP and P-gp in transplantation tumor of A549 tumor-bearing nude mice and without dosage dependence.

关 键 词：补中益气汤 肺癌 多药耐药相关蛋白质类 肺耐药蛋白 P-糖蛋白 A549细胞 

分 类 号：R285[医药卫生—中药学]