Upgrade from aerated static pile to agitated bed systems promotes lignocellulose degradation in large-scale composting through enhanced microbial functional diversity  

作　　者：Hanxia Yu Haoyan Xiao Huiyu Deng Adam Frew Md.Akhter Hossain Wenbing Tan Beidou Xi 

机构地区：[1]School of Geographical Sciences,Fujian Normal University,Fuzhou 350007,China [2]Hawkesbury Institute for the Environment,Western Sydney University,Locked Bag 1797,Penrith,NSW 2751,Australia [3]State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China

出　　处：《Journal of Environmental Sciences》2024年第10期55-66,共12页环境科学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(No.42030704).

摘　　要：Composting presents a viable management solution for lignocellulose-rich municipal solid waste.However,our understanding about the microbial metabolic mechanisms involved in the biodegradation of lignocellulose,particularly in industrial-scale composting plants,remains limited.This study employed metaproteomics to compare the impact of upgrading from aerated static pile(ASP)to agitated bed(AB)systems on physicochemical parameters,lignocellulose biodegradation,and microbial metabolic pathways during largescale biowaste composting process,marking the first investigation of its kind.The degradation rates of lignocellulose including cellulose,hemicellulose,and lignin were significantly higher in AB(8.21%-32.54%,10.21%-39.41%,and 6.21%-26.78%)than those(5.72%-23.15%,7.01%-33.26%,and 4.79%-19.76%)in ASP at three thermal stages,respectively.The AB system in comparison to ASP increased the carbohydrate-active enzymes(CAZymes)abundance and production of the three essential enzymes required for lignocellulose decomposition involving a mixture of bacteria and fungi(i.e.,Actinobacteria,Bacilli,Sordariomycetes and Eurotiomycetes).Conversely,ASP primarily produced exoglucanase andβ-glucosidase via fungi(i.e.,Ascomycota).Moreover,AB effectively mitigated microbial stress caused by acetic acid accumulation by regulating the key enzymes involved in acetate conversion,including acetyl-coenzyme A synthetase and acetate kinase.Overall,the AB upgraded from ASP facilitated the lignocellulose degradation and fostered more diverse functional microbial communities in large-scale composting.Our findings offer a valuable scientific basis to guide the engineering feasibility and environmental sustainability for large-scale industrial composting plants for treating lignocellulose-rich waste.These findings have important implications for establishing green sustainable development models(e.g.,a circular economy based onmaterial recovery)and for achieving sustainable development goals.

关 键 词：Bacterial and fungal community Large-scale composting Lignocellulose degradation Microbial metabolism Metaproteomic analysis 

分 类 号：X705[环境科学与工程—环境工程]