Enhanced remediation of chlorpyrifos-contaminated soil by immobilized strain Bacillus H27  

作　　者：Changrui Liu Shengfang Wen Shuhan Li Yu Tian Lanjun Wang Lusheng Zhu Jun Wang Young Mo Kim Jinhua Wang 

机构地区：[1]National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources,Key Laboratory of Agricultural Environment in Universities of Shandong,College of Resources and Environment,Shandong Agricultural University,Taian 271018,China [2]Department of Civil and Environmental Engineering,Hanyang University,Seongdong-gu,Seoul 04763,Korea

出　　处：《Journal of Environmental Sciences》2024年第10期172-184,共13页环境科学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.42277039 and 42207026);the Natural Science Foundation of Shandong Province,China(No.ZR2022QD098);the Special Funds of Taishan Scholar of Shandong Province,China.

摘　　要：Chlorpyrifos is a pesticide widely used in agricultural production with a relatively long residual half-life in soil.Addressing the problem of residual chlorpyrifos is of universal concern.In this study,rice hull biochar was used as an immobilized carrier to prepare the immobilized strain H27 for the remediation of chlorpyrifos-contamination soil.Soil microorganisms after remediation were investigated by ecotoxicological methods.The immobilized strain H27 had the highest removal rate of chlorpyrifos when 10%bacterial solution was added to the liquid medium containing 0.075-0.109 mm diameter biochar cultured for 22 hr.This study on the removal of chlorpyrifos by immobilized strain H27 showed that the initial concentration of chlorpyrifos in solution was 25mg/L,and the removal rate reached 97.4%after 7 days of culture.In the soil,the removal rate of the immobilized bacteria group increased throughout the experiment,which was significantly higher than that of the free bacteria and biochar treatment groups.The Biolog-ECO test,T-RFLP and RT-RCR were used to study the effects of the soil microbial community and nitrogen cycling functional genes during chlorpyrifos degradation.It was found that ICP group had the highest diversity index among the four treatment groups.The microflora of segment containing 114 bp was the dominant bacterial community,and the dominant microflora of the immobilized bacteria group was more evenly distributed.The influence of each treatment group on ammonia-oxidizing bacteria(AOB)was greater than on ammonia-oxidizing archaea(AOA).This study offers a sound scientific basis for the practical application of immobilized bacteria to reduce residual soil pesticides.

关 键 词：CHLORPYRIFOS Immobilized microorganisms Soil remediation Soil microorganism 

分 类 号：X53[环境科学与工程—环境工程] S15[农业科学—土壤学]