广山楂叶水提取物的急性毒性和遗传毒性  

Acute Toxicity and Genotoxicity of Water Extract from Malus doumeri (Bois.) A.Chev.Leaves

作　　者：叶志青[1] 卢安根[2] 黎颖[2] 叶质萍 张洁宏[4] 黎德全 黄维静 YE Zhiqing;LU Angen;LI Ying;YE Zhiping;ZHANG Jiehong;LI Dequan;HUANG Weijing(Institute of Agriculture and Animal Husbandry Industry Development,Guangxi University,Nanning,Guangxi,530004,China;Guangxi Zhuang Autonomous Region Center for Analysis and Test Research,Nanning,Guangxi,530022,China;Guangxi Zhaoping County Lincheng Ecological Agriculture Professional Cooperatives,Hezhou,Guangxi,546807,China;Guangxi Zhuang Automons Recion Center for Disease Control and Prevention,Nanning,Guangxi,530028,China)

机构地区：[1]广西大学农牧产业发展研究院,广西南宁530004 [2]广西壮族自治区分析测试研究中心,广西南宁530022 [3]广西昭平县林成生态农业专业合作社,广西贺州546807 [4]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《广西科学》2024年第3期492-499,共8页Guangxi Sciences

基　　金：广西重点研发计划项目(桂科AB17292012);广西壮族自治区分析测试研究中心基本业务费项目(GXAT2022ZZ06)资助。

摘　　要：本研究对广山楂Malus doumeri (Bois.)A.Chev.叶水提取物的急性经口毒性和遗传毒性进行试验,考察山楂叶的食用安全性。按《食品安全国家标准食品安全性毒理学评价程序》规定的方法,开展广山楂叶水提取物急性毒性和遗传毒性试验(细菌回复突变试验、哺乳动物红细胞微核试验、小鼠精原细胞染色体畸变试验)。结果表明,广山楂叶水提取物急性毒性的半数致死量LD_(50)大于20 000 mg/kg BW;在8-5 000μg/皿剂量范围内,细菌回复突变试验结果为阴性;在2 500-10 000 mg/kg BW剂量范围内,小鼠骨髓红细胞染色体未见有损伤,精原细胞染色体未致畸变。因此,广山楂叶水提取物属实际无毒级,未观察到其遗传毒性。The edible safety of the water extract from Malus doumeri(Bois.)A.Chev.leaves was examined by acute oral toxicity and genotoxicity tests.According to the experimental method of National Food Safety Standard Procedure for Toxicological Evaluation of Food Safety,the toxicity of M.doumeri(Bois.)A.Chev.leaves was evaluated by acute toxicity and genotoxicity tests(including Ames test,mammalian erythrocyte micronucleus test,and chromosome aberration test of mouse spermatogonia).The results showed that the median lethal dose(LD_(50))of the water extract from M.doumeri(Bois.)A.Chev.leaves was greater than 20000 mg/kg BW.In the range of 8-5000μg/dish,the water extract showed a negative result in the Ames test.Within the dose range of 2500-10000 mg/kg BW,the water extract did not cause damage to mouse bone marrow erythrocytes or lead to chromosome aberration of mouse spermatogonia.According to the results,the water extract of M.doumeri(Bois.)A.Chev.leaves was non toxic and demonstrated no genotoxicity under the test conditions.

关 键 词：广山楂叶 水提取物 急性经口毒性 遗传毒性 

分 类 号：Q958.8[生物学—动物学]