Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,Bursaphelenchus xylophilus  

作　　者：Shuisong Liu Linsong Wang Ronggui Li Mengyu Chen Wenjun Deng Chao Wang Guicai Du Qunqun Guo 

机构地区：[1]College of Life Sciences,Qingdao University,Qingdao 266071,People’s Republic of China

出　　处：《Journal of Forestry Research》2024年第5期191-200,共10页林业研究（英文版）

基　　金：supported by the Shandong Provincial Natural Science Foundation,China(ZR2020MC123);Qingdao Municipal People-benefitting Demonstration Project of Science and Technology,China(23-2-8-cspz-8-nsh).

摘　　要：In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be downregulated.In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system.Here in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite effect.In addition,RNAi of sft-4 changed the female–male ratio and lifespan of PWN.In bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly weakened.These results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.

关 键 词：Black pine PINACEAE Bursaphelenchus xylophilus Sft-4 In situ hybridization RNAI PATHOGENICITY 

分 类 号：S763[农业科学—森林保护学]