侵染甘肃茉莉的茉莉T病毒鉴定及分子进化分析  

Identification and Molecular Evolutionary Analysis of Jasmine Virus T Infecting Jasmine in Gansu Province

作　　者：陈雅寒 路云爽 何志兰 王骆凡 CHEN Yahan;LU Yunshuang;HE Zhilan;WANG Luofan(College of Plant Protection,Gansu Agricultural University,Lanzhou,Gansu 730070)

机构地区：[1]甘肃农业大学植物保护学院,甘肃兰州730070

出　　处：《北方园艺》2024年第17期61-66,共6页Northern Horticulture

基　　金：甘肃农业大学人才启动金资助项目(GAU-KYQD-2019-22);国家自然科学基金资助项目(32160628);甘肃省自然科学基金资助项目(21JR7RA820);甘肃省高等学校创新基金资助项目(2021B-117)。

摘　　要：以表现黄色环纹症状的8份茉莉叶片为试材,采用RT-PCR方法,研究了待测茉莉的病毒种类及茉莉T病毒(jasmine virus T,JaVT)外壳蛋白(coat protein,CP)基因序列遗传多样性,以期为茉莉病毒病的防治提供参考依据。结果表明:4份样品中检测到JaVT,并扩增出4条JaVT的外壳蛋白(CP)基因序列。4个JaVT分离物CP基因间核苷酸序列的一致性为92.20%~95.20%,与NCBI已发表的4个JaVT CP基因核苷酸序列一致性为91.40%~94.00%。基于8个JaVT CP基因全长序列进行系统发育分析,结果表明该研究中的4个JaVT分离物与来自中国的JaVT-MZ962345聚集在一起。8条JaVT全长CP基因序列中检测到3个重组事件。这是甘肃省首次报道茉莉T病毒。Eight jasmine leaves showing yellow ring were used as experimental material.And the pathogen causing jasmine virus disease and the nucleic acid sequence,genetic diversity of jasmine virus T(JaVT)were analyzed by RT-PCR,in order to provide reference for the prevention and treatment of jasmine virus disease.The results showed that JaVT was isolated from 4 samples,and 4 CP gene sequences of JaVT were amplified.The nucleotide sequence identity among the CP genes of the four JaVT isolates was 92.20%-95.20%,and the nucleotide sequence identity between the CP genes of the four JaVT isolates was 91.40%-94.00%with the other 4 JaVT CP genes published in NCBI.Phylogenetic analysis based on the full-length sequences of 8 JaVT CP genes.The results showed that the four JaVT isolates in this study were clustered with JaVT-MZ962345 from China.Three recombination events were detected in 8 JaVT full-length CP gene sequences.

关 键 词：茉莉 JaVT 病毒检测 CP 分子进化分析 

分 类 号：S436[农业科学—农业昆虫与害虫防治]