^(137)Cs-γ射线辐射对大花威灵仙愈伤生长及分化的影响  

Effects of^(137)Cs-γ Ray Radiation on Callus Growth and Differentiation of Clematis courtoisii

作　　者：韩芯 刘超纲[2] 高露璐 张猛超 王淑安[1] 李林芳[1] HAN Xin;LIU Chaogang;GAO Lulu;ZHANG Mengchao;WANG Shu′an;LI Linfang(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing,Jiangsu 210014;Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《北方园艺》2024年第17期103-108,共6页Northern Horticulture

基　　金：江苏省农业科技自主创新资金资助项目(CX(20)3027);江苏省植物资源研究与利用重点实验室开放基金资助项目(JSPKLB202203)。

摘　　要：以大花威灵仙(Clematis courtoisii)的胚根为试材,诱导出胚性愈伤后,采用激素配比和辐射计量分析方法,研究了激素配比和辐射计量对愈伤组织分化的影响,以期为大威灵仙植物组织培养和诱变种质创新相关研究提供参考依据。结果表明:6-BA 2 mg·L^(-1)+NAA 0.05 mg·L^(-1)是诱导愈伤分化的最佳培养基;使用不同剂量(0、30、60、90、120、150、180 Gy)的^(137)Cs-γ射线进行辐射处理,发现60 Gy辐射剂量对愈伤分化率影响最小,120 Gy为大花威灵仙的半致死剂量。^(137)Cs-γ辐射大花威灵仙愈伤组织的适宜剂量范围是60~90 Gy。Taking the embryonic roots of Clematis courtoisii as the experimental material,embryogenic callus was induced.Hormone ratio and radiometric analysis methods were used to study the effects of hormone ratio and radiometric analysis on callus differentiation,in order to provide reference for the research on tissue culture and mutation germplasm innovation of Clematis courtoisii.The results showed that 6-BA 2 mg·L^(-1)+NAA 0.05 mg·L^(-1)was the optimal medium for inducing callus differentiation.By using different doses of^(137)Cs-γrays(0,30,60,90,120,150,180 Gy)for radiation treatment,the radiation dose of 60 Gy had the least impact on callus differentiation rate,and 120 Gy was the semi lethal dose of Clematis courtoisii.The appropriate dose range for^(137)Cs-γradiation in the callus tissue of Clematis courtoisii was 60-90 Gy.

关 键 词：^(137)Cs-γ辐射 大花威灵仙 愈伤分化 

分 类 号：S853.75[农业科学—临床兽医学]