新疆塔额垦区牛羊粪源志贺菌耐药性、毒力基因检测及其致病性分析  

Analysis of Antimicrobial Susceptibility Testing,Virulence Genes,and Pathogenicity of Shigella Spp.Isolated from Cattle and Sheep Feces in Taer Reclamation Area,Xinjiang

作　　者：吴自豪 王雪 刘鹏 彭健 朱婷婷 李静 张秀萍[1] 齐萌[1] 吴静[1] WU Zihao;WANG Xue;LIU Peng;PENG Jian;ZHU Tingting;LI Jing;ZHANG Xiuping;QI Meng;WU Jing(College of Animal Sciences and Technology,Tarim University,Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control of Xinjiang Production&Construction Corps,Alar 843300,China;College of Life Sciences and Technology,Tarim University,Alar 843300,China;Institute of Agricultural Sciences(Institute of Animal Science),9th Division of Xinjiang Production and Construction Corps,Emin 834601,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,阿拉尔843300 [2]塔里木大学生命科学与技术学院,阿拉尔843300 [3]新疆生产建设兵团第九师农业科学研究所(畜牧科学研究所),额敏834601

出　　处：《中国草食动物科学》2024年第5期37-42,共6页China Herbivore Science

基　　金：新疆生产建设兵团科技创新人才计划(2021CB062);新疆生产建设兵团第九师科技计划项目(2021JS001,2022JS008)。

摘　　要：为了解新疆塔额垦区牛羊粪源志贺菌的耐药性、毒力基因携带情况和致病性,对从335份牛羊腹泻粪便中分离鉴定到的32株志贺菌(10株福氏志贺菌和22株宋内志贺菌)进行药敏试验、耐药基因与毒力基因检测、生物被膜形成能力和小鼠致病性检测。结果显示,32株志贺菌对四环素、多西环素和氨苄西林等药物的耐药率较高,分别为87.50%、87.50%和84.38%;29株(90.63%)呈现多重耐药。从分离株中检测到15种耐药基因,其中blaTEM(96.88%)和blaCXT-M(68.75%)基因的检出率较高;检测到14种毒力基因,其中yijP(93.75%)、ibeB(90.63%)和ipaBCD(78.13%)基因的检出率较高。部分志贺菌具有生物被膜形成能力(2/32,6.25%),并引起小鼠死亡。说明新疆塔额垦区牛羊源志贺菌具有较强的耐药性,携带多种耐药基因和毒力基因,具有一定的致病性,在牛羊健康养殖中具有潜在威胁。In order to understand the drug resistance,virulence gene carrying status,and pathogenicity of Shigella from cattle and sheep feces in the Taer reclamation area of Xinjiang,32 Shigella strains(10 Shigella flexneri and 22 Shigella sonnei)isolated and identified from 335 cattle and sheep diarrhea feces were subjected to drug sensitivity testing,resis‐tance gene and virulence gene detection,biofilm formation ability,and mouse pathogenicity testing.The results showed that 32 Shigella strains exhibited high levels of resistance to tetracycline,doxycycline,and ampicillin,which were 87.50%,87.50% and 84.38%,respectively.29 strains(90.63%)were identified as multiple drug-resistant(MDR)strains.A total of 15 resistance genes were identified in Shigella,with blaTEM(96.88%)and blaCXT-M(68.75%)exhibiting high de‐tection rates.Additionally,14 virulence genes were detected in Shigella with yijp(93.75%),ibeB(90.63%)and ipaBCD(78.13%)showing high detection rates.Some of Shigella species exhibited biofilm-forming capability(2/32,6.25%)and induced resulting in mortality of mice.In summary,the Shigella strains isolated from cattle and sheep in the Taer reclamation area of Xinjiang exhibit heightened levels of antibiotic resistance,harbor multiple resistance genes and virulence genes,demonstrate the capacity for biofilm formation,and possess a degree of pathogenicity posing a potential risk to livestock breeding operations.

关 键 词：志贺菌 腹泻 耐药性 毒力基因 牛 羊 

分 类 号：S855.1[农业科学—临床兽医学]