异氟烷短期内可逆的抑制星形胶质细胞葡萄糖摄取  

Isoflurane Reversibly Inhibits Glucose Uptake in Astrocytes in the Short Term

作　　者：郝景茹[1] 宋雨桐 高灿[1] HAO Jingru;SONG Yutong;GAO Can(NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs,Jiangsu Province Key Laboratory of Anesthesiology,Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application,Xuzhou Medical University,Xuzhou Jiangsu 221004)

机构地区：[1]国家药品监督管理局麻醉药品和精神药品研究与评价重点实验室,江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用重点实验室,徐州医科大学,江苏徐州221004

出　　处：《中国科技纵横》2024年第13期138-140,共3页China Science & Technology Overview

基　　金：国家自然科学基金资助项目(82371215)。

摘　　要：本文主要探讨了异氟烷处理后复灌对星形胶质细胞葡萄糖吸收的影响。利用培养的大鼠星形胶质细胞,2%异氟烷孵育6h,分别复灌24h、48h、72h,观察星形胶质细胞的形态;利用CCK-8试剂盒检测星形胶质细胞活性;加入荧光标记6-NBDG,观察星形胶质细胞葡萄糖吸收的变化;运用RT-PCR检测葡萄糖转运体GLuT1的变化。实验结果表明,异氟烷处理后复灌的星形胶质细胞形态无明显差别,细胞活性均不受影响;复灌24h葡萄糖摄取受抑制,48h恢复;复灌24h的GLuT1 mRNA水平降低,48h恢复;异氟烷可抑制星形胶质细胞葡萄糖转运体的表达并导致葡萄糖摄取障碍,但在短期可逆转。To investigate the effects of isoflurane reperfusion on glucose uptake in astrocytes.Cultured rat astrocytes were exposed with 2%isoflurane for 6h,then reperfused for 24h,48h or 72h to observe the morphological and the cell activity of astrocytes.Using 6-NBDG to detect the glucose absorption.Using RT-PCR to detect the glucose transporters GLuT1 mRNA levels.Isoflurane reperfusion have no effect on the morphology and cell activity of astrocytes.Isoflurane reperfusion inhibited glucose uptake and GLuT1 expression at 24h but not 48h and 72h.Isoflurane may inhibit glucose transporters transport expression,resulting in impaired glucose uptake,but it can be reversed in the short term.

关 键 词：异氟烷 葡萄糖摄取 星形胶质细胞 葡萄糖转运体 

分 类 号：R2-03[医药卫生—中医学]