五苓散对骶髓损伤后神经源性膀胱大鼠膀胱组织NGF/TrkA信号通路的影响  

Effect of Wuling Powder(五苓散)on the NGF/TrkA Signaling Pathway in Bladder Tissue of Rats with Neurogenic Bladder after Sacral Cord Injury

作　　者：张琳[1] 王杨 刘铭泽 王振亮[1] ZHANG Lin;WANG Yang;LIU Mingze;WANG Zhenliang(Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046

出　　处：《辽宁中医药大学学报》2024年第10期35-40,F0003,共7页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：郑州市协同创新专项(2023XTCX049)。

摘　　要：目的观察五苓散对骶髓损伤后神经源性膀胱大鼠膀胱组织中神经生长因子(NGF)/及其受体(TrkA)信号通路的影响,探讨五苓散改善骶髓损伤休克期后逼尿肌无反射型膀胱功能的可能机制。方法将60只雌性SD大鼠按随机数字表法分为假手术组(n=10)和造模组(n=50)。假手术组仅咬除椎板不横断脊髓,造模组采用改良的Hassan Shaker脊髓横断法建立骶髓损伤大鼠模型,选取32只造模成功的大鼠随机分为模型组和五苓散高、中、低剂量组,每组8只。中药治疗组从造模后第15天予以五苓散灌胃治疗,1次/d,连续治疗14 d。干预结束后取材,HE染色法观察膀胱组织病理形态学变化,免疫组织化学法检测膀胱组织NGF、TrkA的表达,Western blot法检测NGF、TrkA蛋白表达,实时荧光定量PCR法测定NGF、TrkA mRNA表达。结果模型组和五苓散高、中、低剂量组大鼠膀胱NGF、TrkA阳性表达、蛋白和mRNA相对表达量,与假手术组相比升高(P<0.05,P<0.01);五苓散中、高剂量组膀胱逼尿肌NGF、TrkA阳性表达、蛋白及mRNA表达水平较模型组升高(P<0.05,P<0.01),其中五苓散高剂量组显著升高(P<0.01);与低剂量组比较,五苓散高剂量组的阳性表达、蛋白和mRNA表达量显著升高(P<0.01);与五苓散中剂量比较,高剂量组阳性表达、蛋白和mRNA表达有所增加(P<0.05,P<0.01)。结论五苓散改善骶髓损伤后神经源性膀胱大鼠膀胱功能,其机制可能与五苓散上调膀胱组织NGF、TrkA表达,激活NGF/TrkA信号通路,帮助逼尿肌收缩相关。Objective To observe the effect of Wuling Powder(五苓散)on the nerve growth factor(NGF)/and its receptor(TrkA)signaling pathway in bladder tissue of neurogenic bladder rats with sacral cord injury,and to explore the possible mechanism of Wuling Powder improving the non reflex bladder function of detrusor muscle after shock phase of sacral cord injury.Methods Sixty female SD rats were randomly divided into a sham surgery group(n=10)and a modeling group(n=50)using a random number table method.The modeling group used the improved Hassan Shaker spinal cord transection method to establish a rat model of sacral cord injury.32 successfully modeled rats were randomly divided into a model group and a high,medium,and low dose group of Wuling Powder,with 8 rats in each group.The traditional Chinese medicine treatment group was treated with Wuling Powder by gavage from the 15th day after modeling,once a day,for 14 consecutive days.After the intervention,samples were collected and HE staining was used to observe the pathological changes in the bladder detrusor tissue.Immunohistochemical staining was used to detect the expression of NGF and TrkA in the detrusor muscle.Western blot was used to detect the relative expression levels of NGF and TrkA proteins in the detrusor muscle,and real-time fluorescence quantitative PCR was used to determine the mRNA expression of NGF and TrkA.Results The positive expression of NGF and TrkA,as well as the relative expression of protein and mRNA in the bladder of rats in the model group,high,medium,and low dose groups of Wuling Powder,were higher than those in the sham operation group(P<0.05,P<0.01);The positive expression levels of NGF and TrkA in the bladder detrusor muscle,as well as protein and mRNA expression levels,were higher in the medium and high dose groups of Wuling Powder compared to the model group(P<0.05,P<0.01),with a significant increase in the high-dose group of Wuling Powder(P<0.01);Compared with the low-dose group,the positive expression,protein and mRNA expression levels

关 键 词：五苓散 神经源性膀胱 NGF TRKA 逼尿肌 

分 类 号：R285.5[医药卫生—中药学]