白通汤治疗溃疡性结肠炎的最佳配比及其谱-效关系  

Optimal Ratios and Their Spectral-efficacy Relationship of Baitong Decoction in the Treatment of Ulcerative Colitis

作　　者：吕婧星 王晓仙[2] 张梦欣 高铭淑 孙安妮 刘慷洁 黄传奇[2] 徐宏峰 LYU Jingxing;WANG Xiaoxian;ZHANG Mengxin;GAO mingshu;SUN Anni;LIU Kangjie;HUANG Chuanqi;XU Hongfeng(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Department of Pharmacy,Wuhan No.1 Hospital,Wuhan 430022,China)

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]武汉市第一医院药学部,武汉430022

出　　处：《医药导报》2024年第10期1537-1545,共9页Herald of Medicine

基　　金：湖北省卫健委中医药科研面上项目(ZY2021M009);湖北省科技厅湖北省自然科学基金青年项目(2021CFB246)。

摘　　要：目的基于药效探究白通汤最佳药物配比,明确其谱-效关系,并分析其潜在的质量标志物。方法以葡聚糖硫酸钠(DSS)构建小鼠溃疡性结肠炎(UC)模型,通过评价各组实验动物UC的表观评分、病理学评分和炎症因子变化来判断不同药物配比白通汤的功效。以高效液相色谱(HPLC)法建立不同药物配比白通汤的指纹图谱,通过偏最小二乘回归分析各物质含量与药效之间的关系,确定白通汤潜在质量标志物。结果附子、干姜、葱白质量比为1∶2∶2组别的白通汤缓解UC效果最佳。指纹图谱确定7种配比的14个共有峰,通过偏最小二乘回归分析发现9个峰与缓解UC有关联。结论白通汤治疗UC的最佳药物配比(附子∶干姜∶葱白)为1∶2∶2,通过谱-效关系分析推测白通汤的质量标志物可能为峰2(苯甲酰乌头原碱)、3、5、6、8(新乌头碱)、9(乌头碱)、10(次乌头碱)、13(10-姜酚)、14所代表的物质。Objective To explore the optimal ratio of Baitong decoction based on efficacy,clarify its spectrum-effect relationship,and identify its potential quality markers.Methods An ulcerative colitis(UC)model in mice was established using dextran sulfate sodium.The efficacy of Baitong decoction with varying drug ratios was assessed by evaluating the apparent score,pathological score and inflammatory factor changes of UC in each group of experimental animals.The fingerprints of Baitong decoction with different ratios were established by high performance liquid chromatography(HPLC),and the relationship between the content of each substance and its efficacy was analyzed by partial least squares regression to determine the potential quality markers of Baitong decoction.Results Baitong decoction was most effective in relieving ulcerative colitis when the mass ratio of Fuzi,Ganjiang and Congbai was 1∶2∶2.The fingerprinting identified 14 common peaks across 7 ratios,with 9 peaks were found to be associated with the remission of ulcerative colitis by partial least squares regression analysis.Conclusion The optimal ratio of Fuzi,Ganjiang and Congbai for treating UC is 1∶2∶2.The spectrum-effect relationship analysis suggested that the quality markers of Baitong decoction may be the substances represented by peak 2(benzoylaconine),3,5,6,8(mesaconine),9(aconitine),10(hypaconitine),13(10-gingerol)and 14.

关 键 词：白通汤 附子 干姜 葱白 质量标志物 溃疡性结肠炎 

分 类 号：R285[医药卫生—中药学] R574.1[医药卫生—中医学]