基于网络药理学研究地锦草对糖尿病的作用机制  

Exploring the mechanisms of action of euphorbia humifusa in diabetes based on network pharmacology

作　　者：于武华 桂明明 何平平 苏慧 纪德佳 邓小燕 YU Wuhua;GUI Mingming;HE Pingping;SU Hui;JI Dejia;DENG Xiaoyan(Jiangxi College of Traditional Chinese Medicine,Jiangxi Province,Fuzhou 344000,China)

机构地区：[1]江西中医药高等专科学校,江西省抚州市344000

出　　处：《临床合理用药杂志》2024年第28期1-5,11,共6页Chinese Journal of Clinical Rational Drug Use

基　　金：江西省教育厅科学技术研究项目(GJJ2205914);江西中医药高等专科学校科研项目(2022B03)。

摘　　要：目的使用网络药理学方法分析地锦草对糖尿病产生作用的物质成分及治疗机制。方法借助TCMSP数据库检索地锦草中所含的主要活性成分及其作用靶点并进行筛选,得到地锦草治疗糖尿病的活性成分和作用靶点。筛选数据库GeneCards和OMIM以得到治疗糖尿病的相关基因,与药物活性成分作交集。依据STRING数据库并采用Cytoscape软件分析蛋白质相互作用(PPI)网络,获得核心靶点后通过在线分析网站Metascape进行GO分析和KEGG通路分析。结果共获得地锦草6个主要活性成分与糖尿病有关的作用靶点131个,GO分析结果显示主要与血管生成、抗缺氧、血小板激活、炎性反应等生物过程相关,KEGG富集分析表明关键通路主要集中于癌症通路与炎症相关通路等。结论PPI研究发现TNF-α、IL-6、AKT1均居于地锦草治疗糖尿病相关靶点的前5位,验证了网络药理学预测靶点的准确性。了解了地锦草治疗糖尿病的物质基础和分子机制,研究结果对地锦草的后续开发利用有一定的积极作用。Objective To explore the active compounds in euphorbia humifusa responsible for its therapeutic effects on diabetes and to elucidate the underlying mechanisms based on network pharmacology.Methods Using the TCMSP database,the primary chemical components and target proteins of euphorbia humifusa were retrieved.Through screening,the active ingredients and target proteins of euphorbia humifusa on treating diabetes were identified.GeneCards and OMIM databases were screened to obtain genes related to diabetes treatment,and these genes were intersected with the drug′s active ingredients.The STRING database was used to analyze protein-protein interaction(PPI)networks using Cytoscape software,and core targets were obtained.Subsequently,GO analysis and KEGG pathway analysis were performed using the online analysis platform Metascape.Results This study identified 6 major active compounds in euphorbia humifusa and 131 associated targets related to diabetes.GO analysis revealed that these targets were primarily involved in angiogenesis,anti-hypoxia,platelet activation,and inflammatory response.KEGG enrichment analysis indicated that the key pathways mainly focus on cancer pathways and inflammation-related pathways.Conclusion This study identified TNF-α,IL-6,and AKT1 as central targets of euphorbia humifusa in diabetes treatment,validating the network pharmacology approach.By elucidating the molecular mechanisms underlying these effects,this research provides a strong foundation for future development of euphorbia humifusa-based therapeutics.

关 键 词：糖尿病 地锦草 网络药理学 

分 类 号：R285[医药卫生—中药学]