LncRNA-Scarna10对白藜芦醇促进巨噬细胞M2极化影响的实验研究  

Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages

作　　者：罗剑明 井洁[1] 梁耀丹 张泰程 程冬瑀 姜海行[1] 覃山羽[1] LUO Jianming;JING Jie;LIANG Yaodan;ZHANG Taicheng;CHENG Dongyu;JIANG Haixing;QIN Shanyu(Department of Gastroenterology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院消化内科,南宁530021

出　　处：《广西医科大学学报》2024年第8期1120-1126,共7页Journal of Guangxi Medical University

基　　金：国基自然科学基金资助项目(No.81960119);广西研究生教育创新计划项目(No.YCSW2024270)。

摘　　要：目的:探讨lncRNA-Scarna10(Scarna10)对白藜芦醇(RSV)促进巨噬细胞极化的影响。方法:LPS构建RAW264.7巨噬细胞M1极化模型,RSV干预24 h,设置分组:control组、LPS组、RSV+LPS组,采用实时荧光定量聚合酶链式反应(RT-qPCR)检测Scarna10表达水平,蛋白质免疫印迹法(western blotting)及免疫荧光检测巨噬细胞极化标志物(iNOS、Arg-1、CD206)表达。在RAW264.7细胞中沉默Scarna10,分为Si-NC组、LPS+Si-NC组、RSV+LPS+Si-NC组、RSV+LPS+Si-Scarna10组,检测转染后各组细胞内Scarna10、巨噬细胞极化标志物的表达。结果:经LPS诱导及白藜芦醇干预后,LPS组M1极化标志物iNOS蛋白表达水平升高,RSV+LPS组M2极化标志物Arg-1、CD206蛋白表达水平均升高,iNOS的蛋白表达水平下降,同时Scarna10在RSV+LPS组的表达较LPS组升高;沉默Scarna10后,与RSV+LPS+Si-NC组比较,RSV+LPS+Si-Scarna10组Scarna10表达及Arg-1、CD206蛋白表达水平均下降,iNOS的蛋白表达水平升高。以上各组间的差异均有统计学意义(均P<0.05)。结论:Scarna10在RSV干预后的M1型巨噬细胞中表达升高,沉默Scarna10可以逆转RSV对M1型巨噬细胞向M2型转化的促进作用。Objective:To investigate the effect of lncRNA-Scarna10(Scarna10)on resveratrol(RSV)-induced polarization of macrophages.Methods:M1 polarization model of RAW264.7 macrophages was established by LPS and treated with RSV for 24 hours.The study groups were divided into control group,LPS group and RSV+LPS group.Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)was used to detect the ex-pression of Scarna10,and western blotting and immunofluorescence were used to detect the expression of macro-phage polarization markers(iNOS,Arg-1,CD206).RAW264.7 cells were divided into Si-NC group,LPS+Si-NC group,RSV+LPS+Si-NC group and RSV+LPS+Si-Scarna10 group after silencing Scarna10,and the expression of Scarna10 and macrophage polarization markers in each group after transfection was detected.Results:After LPS induction and resveratrol intervention,the protein expression level of iNOS,a M1 polarization marker,was elevated in the LPS group,the protein expression levels of Arg-1 and CD206,M2 polarization markers,were ele-vated,while the protein expression level of iNOS was decreased in the RSV+LPS group.At the same time,the ex-pression level of Scarna10 in the RSV+LPS group was higher than that in the LPS group.After silencing Scar-na10,compared with the RSV+LPS+Si-NC group,the expression level of Scarna10 and the protein expression levels of Arg-1 and CD206 in RSV+LPS+Si-Scarna10 group were decreased,and the protein expression level of iNOS was elevated.The differences among the above groups were statistically significant(all P<0.05).Conclu-sion:The expression of Scarna10 is upregulated in M1 macrophages following RSV intervention,and silencing Scarna10 can reverse the RSV-induced promotion of M1-to-M2 macrophage polarization.

关 键 词：LncRNA-Scarna10 白藜芦醇 巨噬细胞极化 

分 类 号：R285.5[医药卫生—中药学]