研磨处理可注射牙髓细胞外基质促进牙髓再生  

Injectable dental pulp extracellular matrix prepared by grinding treatment for dental pulp regeneration

作　　者：杨毓茜 李文俊 赵健 陈岗 Yang Yuqian;Li Wenjun;Zhao Jian;Chen Gang(Department of Stomatology,First Affiliated Hospital of Dalian Medical University,Dalian 116011,Liaoning Province,China)

机构地区：[1]大连医科大学附属第一医院口腔科,辽宁省大连市116011

出　　处：《中国组织工程研究》2025年第22期4663-4670,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81600818),项目负责人:陈岗;辽宁省教育厅项目(LJKZ0855),项目负责人:陈岗。

摘　　要：背景:可注射细胞外基质材料用于牙髓组织再生时,首先需要经过胃蛋白酶消化,再与其他基质成分如水凝胶结合使用,而这也意味着天然细胞外基质材料所保留的微环境遭到破坏。目的:探讨研磨处理条件下制备的可注射牙髓细胞外基质材料用于牙髓组织再生的效果。方法:(1)将获取的猪牙来源牙髓组织经过脱细胞处理后制备成牙髓脱细胞基质材料,经过液氮冷冻、高速研磨处理后,制备成可注射牙髓细胞外基质材料,比较可注射牙髓细胞外基质材料和天然牙髓细胞外基质在细胞黏附效率和微结构方面的差异,免疫组化分析可注射牙髓细胞外基质中的细胞外基质蛋白质成分;(2)将接种有牙髓干细胞的可注射牙髓细胞外基质材料注入到牙本质基质材料构建的牙髓腔中,植入裸鼠皮下1,3,5周后取材,观察可注射牙髓细胞外基质材料的体内改建速率及其在裸鼠皮下的异位牙髓组织再生能力。结果与结论:(1)与天然牙髓细胞外基质相比,可注射牙髓细胞外基质材料显著提高了细胞黏附率,并保留了与天然牙髓细胞外基质相似的多孔胶原结构;(2)与天然牙髓细胞外基质相比,免疫组化染色显示可注射牙髓细胞外基质材料中层粘连蛋白、Ⅰ型胶原蛋白和整合素β1的表达没有显著变化,而纤连蛋白的表达显著下降;(3)体内实验显示,可注射牙髓细胞外基质材料具有优异的成血管能力,并促进了牙髓样组织的形成。结果表明,研磨处理的可注射牙髓细胞外基质材料可促进牙髓再生。BACKGROUND:In the preparation of injectable extracellular matrix materials,most of the materials require pepsin digestion followed by combination with other matrix components like hydrogels for pulp tissue regeneration,which also means the destruction of the microenvironment retained by natural extracellular matrix materials.OBJECTIVE:To investigate the effect of injectable dental pulp extracellular matrix prepared by grinding treatment on the regeneration of dental pulp tissue.METHODS:(1)The dental pulp tissue from pig teeth was prepared into acellular dental pulp matrix materials after acellular treatment.After freezing in liquid nitrogen and high-speed grinding,the injectable dental pulp extracellular matrix was prepared.Then,the differences in cell adhesion efficiency and microstructure between the injectable dental pulp extracellular matrix and natural dental pulp extracellular matrix were compared.Immunohistochemical analysis was used to analyze extracellular matrix protein components in injectable dental pulp extracellular matrix.(2)Injectable dental pulp extracellular matrix material inoculated with dental pulp stem cells was injected into the dental pulp cavity constructed from the dentin matrix material.The samples were obtained after 1,3,and 5 weeks of subcutaneous implantation in nude mice to observe the in vivo conversion rate of injectable dental pulp extracellular matrix materials and the regeneration ability of ectopic dental pulp tissue under the skin of nude mice.RESULTS AND CONCLUSION:(1)Compared with the dental pulp extracellular matrix,the injectable dental pulp extracellular matrix significantly improved the cell adhesion rate and retained the porous collagen structure similar to that of natural dental pulp extracellular matrix.(2)Compared with the natural dental pulp extracellular matrix,immunohistochemical staining showed that there was no significant difference in the expression of fibronectin,collagen type I,and integrinβ1 in the injectable dental pulp extracellular matrix,while the exp

关 键 词：研磨 人牙髓干细胞 细胞外基质 可注射 牙髓组织再生 支架材料 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R783.1