纳米金颗粒@介孔二氧化硅修饰钛种植体促进高糖条件下的成骨分化  

Gold nanoparticle@mesoporous silica modified titanium implants promote osteogenic differentiation under high glucose conditions

作　　者：邓云艺 陈世超 罗明东 李若彤 兰小蓉 余科 李广文 Deng Yunyi;Chen Shichao;Luo Mingdong;Li Ruotong;Lan Xiaorong;Yu Ke;Li Guangwen(Department of Implantology,Affiliated Stomatological Hospital,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Oral&Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory,Luzhou 646000,Sichuan Province,China;Institute of Stomatology,Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属口腔医院种植科,四川省泸州市646000 [2]口颌面修复重建和再生泸州市重点实验室,四川省泸州市646000 [3]西南医科大学口腔医学研究所,四川省泸州市646000

出　　处：《中国组织工程研究》2025年第22期4694-4701,共8页Chinese Journal of Tissue Engineering Research

基　　金：四川省科技厅重点研发项目(22YFS0634),项目负责人:兰小蓉;泸州市科技局重点研发计划(面上)项目(2022-SYF-33),项目负责人:李广文;西南医科大学自然科学重点项目(2022ZD015),项目负责人:李广文;西南医科大学附属口腔医院院级重点项目(2022Z01),项目负责人:李广文;四川省自然科学基金面上项目(2024NSFSC0680),项目负责人:李广文。

摘　　要：背景:微纳米结构改性是钛种植体表面处理的热点研究领域。糖尿病高血糖环境会影响钛种植体与骨组织形成稳定的结合,探索通过表面微纳结构改性来提高钛种植体在高糖环境下的成骨活性是有必要的。目的:观察钛表面纳米金颗粒@介孔二氧化硅涂层对体外高糖条件下成骨分化的影响。方法:分别制备纳米金颗粒悬浮液与介孔二氧化硅,将二者按一定比例混合在去离子水中,制备纳米金颗粒@介孔二氧化硅悬浮液。取钛片,分3组处理:光滑组经过水砂纸打磨处理,纳米管组经过水砂纸打磨处理后采用阳极氧化技术制备二氧化钛纳米管涂层,实验组制备二氧化钛纳米管涂层后浸泡于纳米金颗粒@介孔二氧化硅悬浮液中,制备纳米金颗粒@介孔二氧化硅涂层,表征3组钛片表面的微观形貌、亲水性。将大鼠骨髓间充质干细胞接种于3组钛片表面,通过细胞活/死荧光染色及CCK-8实验检测细胞增殖,DAPI/鬼笔环肽染色检测细胞黏附。将大鼠骨髓间充质干细胞接种于3组钛片表面,加入高糖成骨诱导培养基培养,通过碱性磷酸酶与茜素红S染色检测成骨分化。结果与结论:(1)扫描电镜下可见光滑组钛片表面均匀平坦,纳米管组钛片表面排列紧密的二氧化钛纳米管阵列,实验组钛片在二氧化钛纳米管表面及内部均负载纳米金颗粒@介孔二氧化硅;纳米管组、实验组钛片亲水性均优于光滑组。(2)细胞活/死荧光染色结果显示,3组钛片表面的细胞活力均高于90%;CCK-8实验结果显示,实验组细胞增殖率高于光滑组、纳米管组;DAPI/鬼笔环肽染色结果显示,二氧化钛纳米管涂层、纳米金颗粒@介孔二氧化硅涂层更有利于细胞黏附。(3)碱性磷酸酶与茜素红S染色结果显示,实验组钛片表面细胞碱性磷酸酶活性与细胞外基质矿化程度均高于光滑组、纳米管组。(4)结果表明,纳米金颗粒@介孔二氧化硅涂层�BACKGROUND:Titanium surface micro-nano structure modification is a hot research field in titanium implant surface treatment.The diabetic hyperglycemia environment will affect the stable bonding between titanium implant and bone tissue,so it is necessary to explore the surface micro-nano structure modification to improve the osteogenic activity of titanium implant in high glucose environment.OBJECTIVE:To investigate the effect of gold nanoparticle@mesoporous silica nanoparticles(AuNPs@MSNs)coating on osteogenic activity of osteoblasts under high glucose in vitro.METHODS:Gold nanoparticle suspension and mesoporous silica were prepared respectively,and the two were mixed in deionized water in a certain proportion to prepare gold nanoparticle@mesoporous silica suspension.Titanium sheets were taken and divided into three groups for treatment:the smooth group was treated with water sandpaper;the nanotube group was treated with water sandpaper and then anodized to prepare titanium dioxide nanotube coating,and the experimental group prepared titanium dioxide nanotube coating and then immersed in gold nanoparticle@mesoporous silica suspension to prepare gold nanoparticle@mesoporous silica nanoparticles coating.The microscopic morphology and hydrophilicity of the surface of the three groups of titanium sheets were characterized.Rat bone marrow mesenchymal stem cells were inoculated on the surface of the three groups of titanium sheets.Cell proliferation was detected by cell live/dead fluorescence staining and CCK-8 assay.Cell adhesion was detected by DAPI/phalloidin staining.Rat bone marrow mesenchymal stem cells were inoculated on the surface of the three groups of titanium sheets,and high-glucose osteogenic induction medium was added for culture.Osteogenic differentiation was detected by alkaline phosphatase and Alizarin Red S staining.RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that the surface of the titanium sheet in the smooth group was uniform and flat.The titanium dioxide nanotube arrays in the na

关 键 词：纳米金颗粒 介孔二氧化硅 钛表面改性 微纳结构 高糖条件 成骨分化 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R783.6