羊膜细胞外基质与膀胱细胞外基质材料修复大鼠子宫内膜损伤  

Amniotic and bladder extracellular matrix materials in repairing rat endometrial injury

作　　者：孔小娟 马正娇 谈珍瑜[1] 刘鹏[1] Kong Xiaojuan;Ma Zhengjiao;Tan Zhenyu;Liu Peng(First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410000,Hunan Province,China;Department of Obstetrics and Gynecology,Wancheng District Traditional Chinese Medicine Hospital,Nanyang 473000,Henan Province,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南省长沙市410000 [2]南阳市宛城区中医院妇产科,河南省南阳市473000

出　　处：《中国组织工程研究》2025年第22期4731-4739,共9页Chinese Journal of Tissue Engineering Research

基　　金：湖南中医药大学校级科研基金项目(ZYYDX201753),项目负责人;孔小娟。

摘　　要：背景:大量研究已证实,羊膜细胞外基质与膀胱细胞外基质材料均可作为干细胞载体用于子宫内膜损伤的治疗,但是有关两种材料的比较研究相对少见。目的:对比羊膜细胞外基质与膀胱细胞外基质材料作为干细胞载体治疗子宫内膜损伤的差异。方法:采用全骨髓贴壁法分离纯化SD大鼠骨髓间充质干细胞。分别制备SD大鼠羊膜细胞外基质与膀胱细胞外基质材料,然后将骨髓间充质干细胞分别接种于两种材料表面,检测细胞增殖与黏附情况。将40只SD大鼠随机分为4组(n=10),除假手术组外,子宫内膜损伤组、羊膜细胞外基质组、膀胱细胞外基质组均通过机械干预的方式建立子宫内膜损伤模型,分别将羊膜细胞外基质/骨髓间充质干细胞复合物、膀胱基质细胞外基质/骨髓间充质干细胞复合物移植至羊膜细胞外基质组、膀胱细胞外基质组大鼠损伤内膜部位,移植后14,28 d取材检测,苏木精-伊红染色观察大鼠子宫内膜组织形态,酶联免疫吸附法分析子宫内膜组织中碱性成纤维细胞生长因子、胰岛素样生长因子1与血管内皮生长因子水平,免疫组化染色分析子宫内膜组织中波形蛋白与CD34表达。结果与结论:(1)两种细胞外基质材料均有利骨髓间充质干细胞的增殖,相较于膀胱细胞外基质材料,羊膜细胞外基质材料可促进骨髓间充质干细胞的黏附;(2)相较于假手术组,子宫内膜损伤组碱性成纤维细胞生长因子、胰岛素样生长因子1与血管内皮生长因子水平降低(P <0.01),子宫内膜组织形态发育不良,内膜厚度与腺体数量减少,波形蛋白与CD34阳性表达减少(P <0.01);相较于子宫内膜损伤组,羊膜细胞外基质组、膀胱细胞外基质组碱性成纤维细胞生长因子、胰岛素样生长因子1与血管内皮生长因子水平均升高(P <0.05或P <0.01),子宫内膜组织形态明显改善,内膜厚度与腺体数量增加,波形蛋白与CD34阳BACKGROUND:A large number of studies have confirmed that both amniotic extracellular matrix materials and bladder extracellular matrix materials can be used as stem cell carriers for the treatment of endometrial injury,but the difference in effect between the two materials is relatively rare.OBJECTIVE:To compare the difference of amniotic extracellular matrix materials and bladder extracellular matrix materials as stem cell carrier in the treatment of endometrial injury.METHODS:Whole bone marrow adhesion method was used to isolate and purify bone marrow mesenchymal stem cells from SD rats.SD-rat amniotic extracellular matrix materials and bladder extracellular matrix materials were prepared respectively,and then bone marrow mesenchymal stem cells were inoculated on the surface of the two materials to detect cell proliferation and adhesion.Forty SD rats were randomly divided into four groups(n=10 per group).Except the sham operation group,the endometrial injury group,the amniotic extracellular matrix group,and the bladder extracellular matrix group were all established by mechanical intervention.The amniotic extracellular matrix/bone marrow mesenchymal stem cell complex and bladder stromal extracellular matrix/bone marrow mesenchymal stem cell complex were transplanted into the injured endometrial site in the amniotic extracellular matrix group and the bladder extracellular matrix group.Samples were collected and detected 14 and 28 days after transplantation.The morphology of rat endometrial tissue was observed by hematoxylin-eosin staining.The levels of basic fibroblast growth factor,insulin-like growth factor 1,and vascular endothelial growth factor in endometrial tissues were analyzed by enzyme-linked immunosorbent assay.The expression levels of vimentin and CD34 in endometrial tissues were analyzed by immunohistochemical staining.RESULTS AND CONCLUSION:(1)Both kinds of extracellular matrix materials were beneficial to the proliferation of bone marrow mesenchymal stem cells.Compared with bladder extracellular m

关 键 词：羊膜细胞外基质 膀胱细胞外基质 子宫内膜损伤 宫腔粘连 骨髓间充质干细胞 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R713.4