一种用于筛选砷(Ⅲ)适配体和检测砷(Ⅲ)的QCM生物传感器  

A QCM Biosensor for Screening Arsenic(III)Aptamers and Detecting Arsenic(Ⅲ)

作　　者：郑楚君 钱世权 李欣培 颜旭 黄海轩 王雨萱 叶煜玮 袁敏[1] ZHENG Chu-Jun;QIAN Shi-Quan;LI Xin-Pei;YAN Xu;HUANG Hai-Xuan;WANG Yu-Xuan;YE Yu-Wei;YUAN Min(School of Health Science and Engineering,Shanghai Engineering Research Center for Rapid Food Detection,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海食品快速检测工程技术研究中心,上海200093

出　　处：《中国生物化学与分子生物学报》2024年第9期1282-1288,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家重点研发计划“食品营养与安全关键技术研发”重点专项(No.2022YFF1101102)资助。

摘　　要：开发了石英晶体微天平(QCM)-指数富集配体系统进化(SELEX)技术,用于筛选出与砷(Ⅲ)有高亲和力的适配体。设计随机单链DNA文库,以砷(Ⅲ)为靶标固定在巯基乙胺修饰的晶振片上,捕获溶液中处于游离状态的适配体,构建QCM-SELEX筛选方法。经过6轮筛选,对次级文库进行高通量测序,通过QCM共振频率变化计算得出6S1解离系数K d值为0.36μmol/L。将6S1作为探针,构建QCM生物传感器用于检测砷(Ⅲ),该传感器在0.01μmol/L~0.2μmol/L范围内有较好的线性关系,对砷(Ⅲ)检测限为5.2 nmol/L(3σ),具有良好的选择性。A quartz crystal microbalance(QCM)-systematic evolution of ligands by the exponential enrichment(SELEX)technique was developed to screen out aptamers with high affinity for arsenic(Ⅲ).A random single strand DNA library was designed and fixed on the mercaptoethylamine-modified crystal plate with arsenic(Ⅲ)as the target,and the free aptamer was captured in the solution,and the QCM-SELEX screening method was constructed.After 6 rounds of screening,the secondary library was sequenced with high throughput method,and the 6S1 dissociation coefficient K d value was 0.36μmol/L based on QCM resonance frequency.Using 6S1 as a probe,the QCM biosensor was constructed for the detection of arsenic(Ⅲ).The sensor has a good linear relationship in the range of 0.01μmol/L~0.2μmol/L,and the detection limit of arsenic(Ⅲ)is 5.2 nmol/L(3σ),indicatinggood selectivity.

关 键 词：砷(Ⅲ) 适配体 石英晶体微天平(QCM)-指数富集配体系统进化(SELEX)(QCM-SELEX) 测序 

分 类 号：Q584[生物学—生物化学]