拮抗剂方案行短时受精与过夜受精对胚胎发育参数及临床妊娠结局的影响  

Effect of short time insemination and overnight insemination with antagonist protocol on embryo development parameters and clinical pregnancy outcomes

作　　者：张楠 史鸿志 刘聪 ZHANG Nan;SHI Hongzhi;LJU Cong(Department of Reproductive Medicine,Qinhuangdao Materna and Chid Heath Care Hospita,Qinhuangdao 066000,Hebei,China)

机构地区：[1]秦皇岛市妇幼保健院生殖医学科,河北秦皇岛066000

出　　处：《中国性科学》2024年第9期40-44,共5页Chinese Journal of Human Sexuality

基　　金：秦皇岛市科学技术局社会发展与乡村科技科研项目(202301A046)。

摘　　要：目的探讨常规体外受精-胚胎移植(IVF-ET)的拮抗剂方案行短时受精与过夜受精对胚胎发育参数及临床妊娠结局的影响。方法回顾性分析2017年1月至2022年6月秦皇岛市妇幼保健院收治的909例采用拮抗剂方案接受IVF-ET助孕患者的临床资料,按受精方式分为短时受精(短时组,n=424)和过夜受精(过夜组,n=485)。比较两组受精、胚胎发育情况、临床妊娠结局。结果短时组总受精率、正常受精(2PN)受精率低于过夜组,多原核(PN)受精率、囊胚形成率高于过夜组,差异具有统计学意义(P<0.01);两组单原核(1PN)受精率、未见原核卵裂(0PN)受精率、优质胚胎率比较,差异无统计学意义(P>0.05)。两组移植周期率、内膜厚度、平均移植胚胎数、着床胚胎率、临床妊娠率、流产率、活产率比较,差异无统计学意义(P>0.05)。结论拮抗剂方案中过夜受精2PN受精率高于短时受精,但在囊胚形成率方面短时受精更占优势,两者各有优势但并未影响临床结局,过夜受精对拮抗剂方案继发不孕患者是一种值得肯定的受精方式。Objective To explore the effects of the antagonist protocol of conventional in vitro fertilization-embryo transfer(IVF-ET)through short time insemination and overnight insemination on embryo development parameters and clinical pregnancy outcomes.Methods The retrospective analysis was performed based on the clinical data of 909 patients received IVF-ET cycles with antagonist protocol in Qinhuangdao Maternal and Child Health Care Hospital between January 2017 and June 2022.The patients were divided into short time insemination(short time group,n=424)and overnight insemination(overnight group,n=485)according to the methods of fertilization.The fertilization,embryonic development and clinical pregnancy outcome were compared between the two groups.Results The fertilization rate and normal fertilization(2PN)rate of the short time group were lower than those in the overnight group,but the polyspermy rate and blastocyst formation rate were higher than those in the overnight group,with statistically significant differences(P<0.01).There were no statistical difference of the single pronuclear(1PN)fertilization rate,non-pronuclear(0PN)fertilization rate and high quality embryo rate between the two groups(P>0.05).Comparison of the transfer cycle rate,endometrial thickness,average number of transferred embryo,implantation rate,clinical pregnancy rate,abortion rate and live birth rate between the two groups showed no statistical difference(P>0.05).Conclusions In the antagonist protocol,overnight insemination has higher 2PN rate than short time insemination,while short time insemination had more advantage in the blastocyst formation rate.They have their own advantages but do not affect the clinical outcome.For secondary infertility patients using antagonist protocol,the overnight insemination is still a kind of insemination method worthy to be affirmed.

关 键 词：体外受精-胚胎移植 拮抗剂方案 短时受精 过夜受精 

分 类 号：R711[医药卫生—妇产科学]