寿胎丸对自然流产小鼠模型水通道蛋白、胸腺基质淋巴细胞生成素及相关细胞因子表达水平的影响机制研究  

Research on the effect and mechanism of Shoutai Pill on the expression of aquaporin.thymic stromal lyniphopoictin and the related cytokines in a mouse model of spontaneous abortion

作　　者：焦顺 王杏 潘琴[1] 李晖[1] JIAO Shun;WANG Xing;PAN Qin;LI Hui(Department of Obstetrics,Jingzhou Hospital Affiliated to Yangtze University,Jingzhou Central Hospital,Jingzhou 434020,Hubei,China)

机构地区：[1]湖北省荆州市中心医院(长江大学附属荆州医院)产科,湖北荆州434020

出　　处：《中国性科学》2024年第9期121-125,共5页Chinese Journal of Human Sexuality

基　　金：湖北省荆州市科技局科研基金资助项目(2017035)。

摘　　要：目的探究寿胎丸对自然流产小鼠模型水通道蛋白(AQP)、胸腺基质淋巴细胞生成素(TSLP)及相关细胞因子表达水平的影响机制。方法24只无特定病原体级雌性小鼠随机分为正常妊娠组、流产模型组、寿胎丸组、地屈孕酮组,各6只。正常妊娠组和流产模型组采用纯水灌胃,寿胎丸组采用寿胎丸灌胃,地屈孕酮组采用地屈孕酮片灌胃,四组小鼠均行苏木精-伊红(HE)染色和免疫组化反应。比较四组一般情况、流产率、HE染色情况、AQP、TSLP、叉头框转录因子O3(FOXO3)蛋白表达情况。结果四组体重比较,差异无统计学意义(P>0.05)。流产模型组、寿胎丸组、地屈孕酮组流产率均高于正常妊娠组,且寿胎丸组和地屈孕酮组流产率低于流产模型组(P<0.05)。流产模型组、寿胎丸组、地屈孕酮组AQP高于正常妊娠组,TSLP和FOXO3低于正常妊娠组,且寿胎丸组和地屈孕酮组AQP低于流产模型组,TSLP和FOXO3高于流产模型组(P<0.05)。结论给予自然流产小鼠寿胎丸治疗,可显著降低流产率,其机制可能与下调AQP,上调TSLP和FOXO3有关。Objective To explore the effect and mechanism of Shoutai Pill on the expression of aquaporin(AQP),thymic stromal lymphopoietin(TSLP)and the related cytokines in a mouse model of spontaneous abortion.Methods A total of twenty-four female mice of specific pathogen free grade were randomly divided into normal pregnancy group,abortion model group,Shoutai Pill group and dydrogesterone group,with 6 mice in each group.The normal pregnancy group and abortion model group were treated with pure water by gavage,the Shoutai Pill group was treated with Shoutai Pill by gavage,and the dydrogesterone group was treated with dydrogesterone tablets by gavage.The mice in the four groups were all subjected to hematoxylin and eosin(HE)staining and immunohistochemical staining.The general condition,abortion rate,HE staining condition,AQP,TSLP and forkhead box transcription factor O3(FOXO3)protein expression were compared among the four groups.Results There was no significant difference in the body weight among the four groups(P>0.05).The abortion rates in the abortion model group,Shoutai Pill group and dydrogesterone group were all higher than that in the normal pregnancy group,and the abortion rates in the Shoutai Pill group and dydrogesterone group were lower than that in the abortion model group(P<0.05).AQP in the abortion model group,Shoutai Pill group and dydrogesterone group was higher than that in the normal pregnancy group,and their TSLP and FOXO3 were lower than those in the normal pregnancy group.AQP in the Shoutai Pill group and dydrogesterone group was lower than that in the abortion model group,while their TSLP and FOXO3 were higher than those in the abortion model group(P<0.05).Conclusions Treating the spontaneous abortion mice with Shoutai Pill can significantly reduce the abortion rate,and its mechanism may be related to the down-regulation of AQP and the up-regulation of TSLP and FOXO3.

关 键 词：寿胎丸 自然流产 水通道蛋白 胸腺基质淋巴细胞生成素 

分 类 号：R271[医药卫生—中医妇科学]