滤泡调节性T细胞、滤泡辅助性T细胞及其比值在梅毒血清固定患者外周血中的表达及预测价值  

Expression and predictive value of follicular regulatory T cells,follicular helper T cells,and their ratios in peripheral blood of patients with syphilis sero-fixation

作　　者：王涧 沈惠良 黄佳 夏莹烨 WANG Jian;SHEN Huiliang;HUANG Jui;XIA Yingye(Department of Dermatology,Zhejiang Provincial Dermatology Hospital,Huzhou 313200,Zhejiang,China;Medical Laboratory,Zhejiang Provincial Dermatology Hospital,Huzhou 313200,Zhejiang,China;Department of Nursing,Zhejiang Provincial Dermatology Hospital,Huzhou 313200,Zhejiang,China)

机构地区：[1]浙江省皮肤病医院皮肤科,浙江湖州313200 [2]浙江省皮肤病医院检验科,浙江湖州313200 [3]浙江省皮肤病医院护理部,浙江湖州313200

出　　处：《中国性科学》2024年第9期125-129,共5页Chinese Journal of Human Sexuality

基　　金：浙江省医药卫生科技计划项目(2023KY656);湖州市科技计划项目(2020GY51)。

摘　　要：目的分析梅毒血清固定患者外周血滤泡调节性T细胞(Tfr细胞)、滤泡辅助性T细胞(Tfh细胞)及其比值的表达水平,探讨其与梅毒血清固定的相关性及预测价值。方法选取2020年1月至2022年12月浙江省皮肤病医院收治的30例梅毒血清固定患者(梅毒血清固定组)、30例梅毒血清转阴患者(梅毒血清转阴组)作为研究对象。另选取同期30例健康体检者设为健康对照组。利用流式细胞术检测三组外周血Tfh、Tfr细胞水平及Tfr细胞/Tfh细胞比值,分析Tfh、Tfr细胞水平变化与血清甲基胺红不加热血清试验(TRUST)滴度的相关性,绘制受试者工作特征(ROC)曲线评估Tfr细胞水平、Tfr细胞/Tfh细胞比值对梅毒血清固定的预测价值。结果三组Tfh细胞水平比较,差异无统计学意义(P>0.05);梅毒血清固定组Tfr细胞水平、Tfr细胞/Tfh细胞比值均高于梅毒血清转阴组和健康对照组,差异具有统计学意义(P<0.05)。Tfh、Tfr细胞水平与TRUST滴度无显著相关性(r=0.217、0.225,P>0.05)。ROC曲线结果显示,Tfr细胞水平、Tfr细胞/Tfh细胞比值的曲线下面积(AUC)分别为0.707、0.692(P<0.05)。结论梅毒血清固定患者存在Tfh、Tfr细胞的平衡失调,Tfr细胞水平、Tfr细胞/Tfh细胞比值可作为梅毒血清固定的预测指标。Objective To analyze the expression levels of peripheral blood follicular regulatory T cells(Tfr cells),follicular helper T cells(Tfh cells),and their ratios in patients with syphilis sero-fixation,and to explore their correlation and predictive value with syphilis sero-fixation.Methods A total of 30 patients with syphilis sero-fixation(syphilis sero-fixation group)and 30 patients with syphilis-negative(syphilis-negative group)admitted to Zhejiang Provincial Dermatology Hospital from January 2020 to December 2022 were selected as study subjects.In addition,30 healthy medical check-up people were selected as the healthy control group during the same period.Flow cytometry was used to detect the peripheral blood Tfh cells and Tfr cells levels and Tfr cells/Tfh cells ratio in the three groups,and correlation between the changes in Tfh cells and Tfr cells levels and the serum toluidine red unheated serum test(TRUST)titer were analyzed.The receiver operating characteristic(ROC)curve were drawn to assess the predictive value of the Tfr cells levels and Tfr cells/Tfh cells ratio for syphilis sero-fixation.Results There was no significant difference in the levels of Tfh cells among the three groups(P>0.05),whereas the level of Tfr cells and the Tfr cells/Tfh cells ratio were higher in the syphilis sero-fixation group than those of syphilis-negative group and healthy control group,and the differences were statistically significant(P<0.05).In addition,there were no significant correlation between Tfh cells,Tfr cells levels and TRUST titer(r=0.217,0.225,P>0.05).The results of ROC curve showed that the area under curve(AUC)of Tfr cells level and Tfr cells/Tfh cells ratio were 0.707 and 0.692,respectively(P<0.05).Conclusions There is a Tfr cells and Tfh cells imbalance in patients with syphilis sero-fixation,and Tfr cells levels and Tfr cells/Tfh cells ratio can be used as predictors of syphilis sero-fixation.

关 键 词：滤泡调节性T细胞 滤泡辅助性T细胞 梅毒血清固定 

分 类 号：R759[医药卫生—皮肤病学与性病学]