栽培花生籽仁面积和周长性状的QTL定位分析  

Genetic Mapping for Seed Area and Seed Perimeter in Cultivated Peanut(Arachis hypogaea L.)

作　　者：钟文[1] 胡晓辉[2] 杨国群 王素雯 苗华荣[2] 张胜忠 陈静[2] 王菲菲 ZHONG Wen;HU Xiao-hui;YANG Guo-qun;WANG Su-wen;MIAO Hua-rong;ZHANG Sheng zhong;CHEN Jing;WANG Fei-fei(Shandong Provincial Seed Managemen Station,Jinan 250100,China;Shandong Peanut Research Institute,Qingdao 266100,China;The Peo ple's Government of Chenhu Town,Boxing County,Boxing 256500,China;Dongcheng Sub-district Office of Boxing County,Boxing 256500,China)

机构地区：[1]山东省种子管理总站,山东济南250100 [2]山东省花生研究所,山东青岛266100 [3]博兴县陈户镇人民政府,山东博兴256500 [4]博兴县城东街道办事处,山东博兴256500

出　　处：《花生学报》2024年第3期1-8,共8页Journal of Peanut Science

基　　金：国家重点研发计划(2023YFD1202800);山东省自然科学基金项目(ZR2022MC045)。

摘　　要：籽仁面积和周长是影响花生籽仁大小和外观的重要性状。本研究以‘鲁花11号’ב06B16’构建的重组自交系(recombinant inbred line,RIL)群体为试验材料,对花生籽仁面积和籽仁周长在4个环境进行性状考察及QTL定位分析。基于表型数据和高密度遗传图谱信息,在A04和B06染色体上定位到15个与籽仁面积和籽仁周长性状相关的QTL,其中8个与籽仁面积相关,7个与籽仁周长相关。控制籽仁面积和周长的主效位点qSAB06.1d和qSPB06a在多个不同环境均能检测到,表型贡献率为9.52%~33.78%,其增效等位基因均来自鲁花11号,共定位在B06染色体138.53~140.76 Mb区域内。对主效位点qSAB06.1d/qSPB06a进行功能注释,鉴定到3个编码转运蛋白、1个编码ABA受体PYL和1个编码钙调磷酸酶的基因,它们可能是调控花生籽仁面积和周长的候选基因。本研究将为花生产量和外观性状的精细定位和遗传改良奠定理论基础。Seed area and perimeter are important traits affecting the size and appearance of peanut seed.In this study,a recombinant inbred line(RIL)population derived from Luhua11×06B16 was used to conduct QTL mapping analysis of seed area(SA)and seed perimeter(SP)in four different environments.Based on phenotypic data and a high-density genetic map,a total of 15 QTLs associated with SA and SP were identified on chromosomes A04 and B06,including 8 QTLs associated with SA and 7 QTLs associated with SP.The major stable QTL qSAB06.1d and qSPB06a were identified on linkage group B06 associated with SA and SP explaining 9.52%to 33.78%phenotypic variations,locating on the region of 138.53-140.76 Mb on chromosome B06 with Luhua11 allele contributing to the traits.Gene annotation of the major QTL region was performed and identified three transport proteins,an ABA receptor PYL,and a calcium-dependent phosphatase,which may be candidate genes for peanut SA and SP.This study lays a theoretical foundation for the fine mapping and genetic improvement of peanut yield and appearance traits.

关 键 词：花生 籽仁面积 籽仁周长 QTL定位 

分 类 号：Q343.17[生物学—遗传学] S565.2[农业科学—作物学]