基于BSA-seq技术定位花生种皮颜色基因  

Mapping of Candidate Testa Color Genes in Peanuts Based on BSA-Seq

作　　者：王恩琪 殷祥贞 甄萍萍 姜骁 潘丽娟[2] 陈娜[2] 许静 赵旭红 梁成伟[1] 迟晓元[2] WANG En-qi;YIN Xiang-zhen;ZHEN Ping-ping;JIANG Xiao;PAN Li-juan;CHEN Na;XU Jing;ZHAO Xu-hong;LIANG Cheng wei;CHI Xiao-yuan(College of Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;Shandong Peanut Research Institute,Qingdao 266100,China;Agricultural Technology Extension Center of Linyi County,Linyi 251500,China)

机构地区：[1]青岛科技大学生物工程学院,山东青岛266042 [2]山东省花生研究所,山东青岛266100 [3]临邑县农业技术推广中心,山东临邑251500

出　　处：《花生学报》2024年第3期14-20,共7页Journal of Peanut Science

基　　金：新疆维吾尔自治区重大科技专项(2022A02008-3);泰山学者工程(tsqn202312292);财政部和农业农村部:国家现代农业产业技术体系(CARS-13);山东省自然科学基金(ZR2021QC172,ZR2023QC146);山东省重点研发计划(农业良种工程)(2022LZGC007);山东省重点研发计划(乡村振兴科技创新提振行动计划)(2022TZXD0031);山东省农业科学院农业科技创新工程(CXGC2023F20);山东省农业科学院齐鲁农科英才工程(创新类)。

摘　　要：花生种皮颜色是花生重要性状,同时种皮颜色和黄酮类化合物例如花青素的含量有着密切关联。定位与种皮颜色相关的基因对培育和开发新品种,提高花生营养成分有重要作用。本研究以紫色种皮花生‘T371’和粉色种皮花生‘T34’为亲本进行杂交并构建F_2分离群体。基于高通量测序技术,在亲本和分离群体中获得了312.96 G高质量的Clean Reads数据,采用BSA-seq方法定位到1个SNPs和InDels关联区域交集,区间长度为7.61 Mb,位于12号染色体上,区间内有675个基因,其中非同义突变基因14个,移码突变基因3个。根据基因注释结果推测arahy.AFLD8J、arahy.26781N、arahy.YQ76T0、arahy.1KM4NQ、arahy.X3FWT9可能是调控种皮颜色的候选基因。本研究为挖掘花生种皮颜色遗传相关基因和开发新品种提供了一定的理论基础。The testa color of peanuts is an important agronomic trait,which is closely related to the content of flavonoids,such as anthocyanins.Cloning and application of genes related to testa color are crucial for breeding and developing new peanut varieties with improved nutritional properties.In this study,the peanut with purple testa color'T371'and the peanut with pink testa color'T34'were used as parents to construct an F_2 segregating population.Based on high-throughput sequencing technology,312.96 G of high-quality Clean Reads data were obtained in both parental and segregating populations.The BSA-seq approach was applied to identify a main SNPs and InDels regulatory correlation region with interval lengths of 7.61 Mb on chromosome 12,which contained 675 genes,among which 14 genes were found with non-synonymous mutation and 3 genes were detected with frame shift mutation.Based on gene annotation,arahy.AFLD8J,arahy.26781N,arahy.YQ76T0,arahy.1KM4NQ and arahy.X3FWT9 may be candidate genes for regulating testa color.This study is helpful for cloning genes related to peanut testa color and breeding new varieties.

关 键 词：花生 BSA-seq 种皮颜色 基因定位 

分 类 号：Q343.17[生物学—遗传学] S565.2[农业科学—作物学]