橘小实蝇对高效氯氰菊酯、敌百虫和阿维菌素的交互抗性  

Cross-resistance of Bactrocera dorsalis(Diptera:Tephritidae)to beta-cypermethrin,trichlorfon and abamectin

作　　者：李豆豆 姚其 齐易香 陆永跃 LI Dou-Dou;YAO Qi;QI Yi-Xiang;LU Yong-Yue(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Plant Protection Institute,Hainan Academy of Agricultural Sciences,Haikou 570100,China)

机构地区：[1]华南农业大学植物保护学院,广州510642 [2]海南省农业科学院植物保护研究所,海口570100

出　　处：《昆虫学报》2024年第8期1075-1085,共11页Acta Entomologica Sinica

基　　金：国家重点研发计划项目(2021YFC2600404);广东省现代农业产业共性关键技术研发创新团队建设项目(2023KJ134);广州市重点研发计划项目(2023B04J0154)。

摘　　要：【目的】目前化学防治是果树重大害虫橘小实蝇Bactrocera dorsalis的主要防治措施。本研究旨在明确橘小实蝇高效氯氰菊酯、敌百虫和阿维菌素抗性品系成虫对高效氯氰菊酯、敌百虫和阿维菌素3种杀虫剂的抗性,分别对其他2种杀虫剂的交互抗性及其抗性遗传规律,为果园轮换用药开展防治和抗性治理等工作提供理论支持。【方法】从野外采集橘小实蝇幼虫,在实验室内饲养52代以上,应用药膜法测定成虫对杀虫剂的敏感度;采用群体筛选法逐代抗性汰选至52代,建立橘小实蝇抗药性品系;采用药膜法测定橘小实蝇高效氯氰菊酯抗性品系、敌百虫抗性品系和阿维菌素抗性品系成虫分别对另外2种杀虫剂的交互抗性水平;将高效氯氰菊酯抗性品系、敌百虫抗性品系和阿维菌素抗性品系成虫进行正反两两杂交,通过对各杂交处理F1代成虫进行生物测定以判定抗性遗传规律。【结果】研究发现橘小实蝇高效氯氰菊酯、抗敌百虫和阿维菌素抗性品系成虫分别对另外两种杀虫剂均存在一定程度的交互抗性。其中,高效氯氰菊酯抗性品系成虫对敌百虫存在中等水平的交互抗性,抗性倍数(resistance multiple, Rm)为15.61;对阿维菌素则为低水平交互抗性,Rm为6.67;敌百虫和阿维菌素对高效氯氰菊酯抗性品系成虫的致死中浓度(median lethal concentration, LC50)值分别为54.58和25.30 mg/L。敌百虫抗性品系成虫对高效氯氰菊酯和阿维菌素均存在低水平交互抗性,Rm分别为9.15和6.18;高效氯氰菊酯和阿维菌素对敌百虫抗性品系成虫的LC50值分别为43.12和23.35 mg/L。阿维菌素抗性品系成虫对高效氯氰菊酯和敌百虫同样存在低水平交互抗性,Rm分别为8.74和3.58;高效氯氰菊酯和敌百虫对阿维菌素抗性品系的LC50值分别为41.18和12.52 mg/L。橘小实蝇高效氯氰菊酯抗性品系与敌百虫抗性品系进行正反交时其F1代成虫的�【Aim】At present,chemical control is the main measure in controlling Bactrocera dorsalis,a major pest of fruit trees.This study aims to determine the resistance of the adults of the beta-cypermethrin-,trichlorfon-and abamectin-resistant strains of B.dorsalis to three insecticides,namely beta-cypermethrin,trichlorfon and abamectin,their cross-resistance to the other two insecticides and the genetic patterns of their resistance,so as to provide the theoretical support for the chemical rotation and resistance management in orchards.【Methods】B.dorsalis larvae were collected from the fields and reared in the laboratory for more than 52 generations.The sensitivity of adults to insecticides was measured by the drug film method.The population screening method was used in the resistance selection for each generation up to 52 generations to establish the resistant strains of B.dorsalis.The drug film method was used to determine the cross-resistance of adults of the beta-cypermethrin-,trichlorfon-and abamectin-resistant strains,respectively,to the other two insecticides.Pairwise reciprocal crosses were conducted among adults of the beta-cypermethrin-,betatrichlorfon-and abamectin-resistant strains,and the bioassay against the adults of the F 1 generations from the above crossing treatments was conducted to reveal the genetic patterns of the resistance.【Results】It was found that the adults of the beta-cypermethrin-,trichlorfon-and abamectin-resistant strains of B.dorsalis all exhibited some degree of cross-resistance to the other two insecticides.Among them,the adults of the beta-cypermethrin-resistant strain had a moderate level of cross-resistance to trichlorfon,with the resistance multiple(Rm)of 15.61,and showed a low-level cross-resistance to abamectin,with the Rm of 6.67.The median lethal concentration(LC 50)values of trichlorfon and abamectin to the beta-cypermethrin-resistant strain were 54.58 and 25.30 mg/L,respectively.The adults of the trichlorfon-resistant strain had low levels of cross-resistance to bo

关 键 词：橘小实蝇 高效氯氰菊酯 敌百虫 阿维菌素 交互抗性 

分 类 号：Q965.9[生物学—昆虫学]