变温下暗期LED光照对丝棉木金星尺蛾生命活动节律的影响  

Effects of LED light irradiation in scotophase on the rhythmicity in life activities of Calospilos suspecta(Lepidoptara:Geometridae)at variable temperatures

作　　者：程禹朦 龚慧蓉 罗园霞 朱地福 梁嘉瑶 周舒昱 邹可豪 涂小云[1] CHENG Yu-Meng;GONG Hui-Rong;LUO Yuan-Xia;ZHU Di-Fu;LIANG Jia-Yao;ZHOU Shu-Yu;ZOU Ke-Hao;TU Xiao-Yun(College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China)

机构地区：[1]江西师范大学生命科学学院,南昌330022

出　　处：《昆虫学报》2024年第8期1106-1114,共9页Acta Entomologica Sinica

基　　金：国家自然科学基金地区项目(31960558);江西省教育厅科学技术研究项目(GJJ2200307)。

摘　　要：【目的】探明变温下暗期给予不同LED光源对丝棉木金星尺蛾Calospilos suspecta化蛹、羽化、交配、产卵和卵孵化活动节律的影响。【方法】在实验室条件下以光周期14L∶10D为对照组,以暗期分别给予10 h红色LED和10 h黄色LED 2种不同光源照射为处理组(分别记为14L∶10R和14L∶10Y),分别对丝棉木金星尺蛾的化蛹、羽化、交配、产卵和卵孵化进行连续5 d的观察,每小时观察1次,计算化蛹率、羽化率、交配率、产卵率和卵孵化率,并比较这些参数在不同试验组间光期或暗期差异以及各组内光期与暗期间的差异。【结果】红色和黄色LED光照射下丝棉木金星尺蛾化蛹不具明显的节律性,14L∶10D组和14L∶10Y组的化蛹主要发生在光期,化蛹高峰分别在7:00-8:00和18:00-19:00;14L∶10R组主要在暗期化蛹,化蛹高峰在22:00-23:00;但暗期不同LED光处理对平均单时段化蛹率无显著影响。红色和黄色LED光照射下羽化节律亦不明显,14L∶10D组和14L∶10R组的雌雄成虫羽化主要发生在光期,14L∶10Y组雌雄成虫的羽化主要发生在暗期;14L∶10D组和14L∶10R组羽化高峰均在光期开启的第1-2小时;暗期LED光处理显著影响雌成虫平均单时段羽化率,且14L∶10D组光期雌成虫平均单时段羽化率显著高于暗期;暗期两个LED光处理对雄成虫平均单时段羽化率影响不显著。交配行为具有明显的节律性,各组交配行为均主要发生在暗期,交配高峰在光期开启前的1-2 h(4:00-6:00);14L∶10D组和14L∶10Y组暗期平均单时段交配率显著高于光期。产卵亦具有明显的节律性,14L∶10D组和14L∶10R组产卵高峰及4L∶10Y组产卵次高峰均发生在暗期20:00-21:00, 14L∶10Y组产卵高峰在16:00-17:00;暗期不同LED光处理对平均单时段产卵率均无显著影响。卵孵化具有明显的节律性,各组孵化高峰均在光期开始的第1小时;暗期不同LED光处理对平均单时段卵孵化率影响�【Aim】To explore the effects of different LED light sources in scotophase at variable temperatures on the rhythmicity in pupation,eclosion,mating,oviposition and egg hatching activities of Calospilos suspecta.【Methods】Under laboratory conditions,the pupation,eclosion,mating,oviposition and egg hatching of C.suspecta were observed every hour for 5 d in the control group(under the photoperiod of 14L∶10D)and two treatment groups subjected to 10-h red LED light and 10-h yellow LED light irradiation,respectively,in scotophase(represented by 14L∶10R and 14L∶10Y,respectively).The rates of pupation,eclosion,mating,oviposition and egg hatching were calculated,and the differences in these parameters in photophase or scotophase among experimental groups,and between photophase and scotophase within each group were compared.【Results】The pupation of C.suspecta didn’t show obvious circadian pattern under red or yellow LED light irradiation.Pupation in 14L∶10D and 14L∶10Y groups mainly occurred in photophase,and the peak of pupation occurred at 7:00-8:00 and 18:00-19:00,respectively.Pupation in 14L∶10R group mainly occurred in scotophase,and the peak of pupation occurred at 22:00-23:00.However,different LED light treatments in scotophase didn’t significantly affect the pupation rates per hour.The eclosion didn’t show obvious circadian pattern under red or yellow LED light irradiation.Eclosion of female and male adults in 14L∶10D and 14L∶10R groups mainly occurred in photophase,while that in 14L∶10Y group mainly occurred in scotophase.The peak of eclosion in 14L∶10D and 14L∶10R groups occurred on the 1st-2nd hour of photophase.The eclosion rates of female adults per hour in scotophase were significantly affected by LED light treatments.In 14L∶10D group,the eclosion rates of female adults per hour in photophase were significantly higher than those in scotophase.However,two different LED light treatments in scotophase didn’t significantly affect the eclosion rates of male adult per hour.Th

关 键 词：丝棉木金星尺蛾 LED光照 生命活动 昼夜节律 光期 暗期 

分 类 号：Q968[生物学—昆虫学]