蔓生百部中特征性成分原百部碱的考察及其质检探讨  

Investigation and Quality Inspection of Protostemon,a Characteristic Component of Stemona Japonica

作　　者：陈蓓 谭成勇 KONGKIATPAIBOON Sumet 蔡祥海[2] CHEN Bei;TAN Chengyong;KONGKIATPAIBOON Sumet;CAI Xianghai(Bengbu Hospital of Traditional Chinese Medicine,Bengbu 233000,China;Key Laboratory of Phytochemistry and Natural Medicine,Kunming Institute of Botany,CAS,Kunming 650201,China;Drug Discovery and Development Center,Office of Advanced Science and Technology,Thammasat University,Pathum Thani 12121,Thailand)

机构地区：[1]蚌埠市中医医院,安徽蚌埠233000 [2]中国科学院昆明植物研究所植物化学与天然药物实验室,云南昆明650201 [3]Drug Discovery and Development Center,Office ofAdvanced Science and Technology,Thammasat University,Pathum Thani 12121,Thailand

出　　处：《云南中医药大学学报》2024年第4期41-46,共6页Journal of Yunnan University of Chinese Medicine

基　　金：Bualuang ASEAN Chair Professor Fund,泰国。

摘　　要：目的考察蔓生百部中特征性成分原百部碱的稳定性,并分析其在蔓生百部贮存、使用中的变化,探索百部药材的质量控制方法。方法以乙腈-水为流动相,采用HPLC-DAD、C18分析柱梯度分析原百部碱在三氯甲烷、丙酮、甲醇等溶剂中的变化情况,并考察甲醇冷浸提取、水回流提取不同贮存时间的蔓生百部特征成分的变化。结果原百部碱在三氯甲烷、丙酮等溶剂中不同程度地转变成异原百部碱、狭叶百部碱和异狭叶百部碱,在甲醇溶剂中转变减少;随着储存时间的增加,蔓生百部中原百部碱含量下降,而狭叶百部碱和异狭叶百部碱含量增加。结论原百部碱的稳定性较差,且原百部碱是蔓生百部的主要有效成分之一,可能导致蔓生百部在贮存和使用过程易于药效的降低,中药百部尚需制定定量的检测标准。Objective Investigate the stability of protostemonine,a characteristic constitution of Stemona japonica,and analyze its changes in the storage and use,and explore the quality control of Baibu materials.Methods Using acetonitrile-water as mobile phase,gradient analysis on HPLC-DAD coupling C18 column was used to compare the changes of protostemonine in trichloromethane,acetone,methanol and other solvents,and to investigate the changes of the characteristic component of Stemona japonica extracted by methanol immersion and water reflux extracts at different storage times.Results In the solvents of trichloromethane,acetone,protostemonine was apt to change into isoprotostemonine,maistemonine and isomaistemonine.However,the transformation was greatly reduced in the methanol solvent.It was observed that the content of protostemonine decreased with the increase of storage time,but the content of maistemonine and isomaistemonine increased.Conclusion The stability of protostemonin was poor,and it was one of the main active components of Stemona japonica,which may lead to the decrease of efficacy of Baibu during storage and use.The quantitative testing standard of Baibu is still needed.

关 键 词：蔓生百部 原百部碱 稳定性 质量控制 

分 类 号：R285[医药卫生—中药学]