利用多组学方法探究湖羊肠道菌群-脑肠轴互作关系  

Interactions of Microbiota-gut-brain Axis of Hu Sheep via Multiomics

作　　者：丁晓宁 李可瑶 甘雅婷 庞硕 李哲晨 谢贤杰 甘乾福[1] 方绍明 DING Xiaoning;LI Keyao;GAN Yating;PANG Shuo;LI Zhechen;XIE Xianjie;GAN Qianfu;FANG Shaoming(College of Animal Sciences(College of Bee Science),Fujian Agricultural and Forestry University,Fuzhou,Fujian 35002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福建福州350002

出　　处：《家畜生态学报》2024年第9期7-15,90,共10页Journal of Domestic Animal Ecology

基　　金：福建省自然科学基金项目(2020J01537);福建省科技厅星火项目(2019S0036);福建省科技厅引导性项目(2019N0069);福州市科技计划项目(2021-N-129)。

摘　　要：肠道菌群-脑肠轴可参与新陈代谢、免疫应答和行为反应等不同生理过程,是影响生长和健康的重要因素,本研究利用多组学方法探究了湖羊肠道菌群-脑肠轴互作关系。16S-rRNA基因测序分析结果显示,厚壁菌门、拟杆菌门及克里斯腾森菌属-R7类群、肠球菌属、颤螺菌科-UCG002、普雷沃氏菌科-UCG004等菌属是湖羊肠道菌群中的主要类群。整合结肠和脑组织转录组测序分析,发现雌激素信号通路可能是连接湖羊肠道菌群-脑肠轴的关键功能途径,而真杆菌属细菌和瘤胃球菌属细菌可能是调控湖羊肠道菌群-脑肠轴的关键细菌。本研究结果能为在生产实践中通过营养干预调控肠道菌群-脑肠轴而改善湖羊性情和行为习性、提高生产性能提供基础数据。The microbiota-gut-brain axis is involved in different physiological processes such as metabolism,immune responses,and behavioral responses which exerts important effects on health and growth.Herein,the interactions of microbiota-gut-brain axis of Hu sheep was investigated by multiomics in present study.The results of 16S rRNA gene sequencing analysis showed that Firmicutes,Bacteroidetes,Christensenellaceae_R-7_group,Enterococcus,Oscillospiraceae_UCG-002,and Prevotellaceae_UCG-004 were the dominant microbial taxa in the gut microbiota of Hu sheep.In combination with the colon and brain transcriptome analysis,it is found that estrogen signaling pathway may be the key functional pathway that contributes to the establishment of the microbiota-gut-brain axis of Hu sheep.Additionally,it was also found that Eubacterium and Ruminococcus may be the key bacteria which could modulate the microbiota-gut-brain axis of Hu sheep.These findings could provide basic knowledge for improving the temperament,behavior,and production performances of Hu sheep through modulating the microbiota-gut-brain axis by nutritional intervention.

关 键 词：湖羊 肠道菌群 脑肠轴 16S rRNA基因测序 转录组测序 

分 类 号：S826.2[农业科学—畜牧学]