Seeking the identity of an enigmatic moss by embracing phylogenomics  

作　　者：Pablo Aguado-Ramsay Tamara Villaverde Ricardo Garilleti J.Gordon Burleigh Stuart F.McDaniel Maren Flagmeier Jurgen Nieuwkoop Arno van der Pluijm Florian Hans Francisco Lara Isabel Draper 

机构地区：[1]Departamento de Biologia,Facultad de Ciencias,Universidad Autonoma de Madrid,C.Darwin,2,Madrid 28049,Spain [2]Doctoral Program in Biology,Doctoral School(EDUAM),Universidad Autonoma de Madrid,C.Francisco Tomas y Valiente,2,Madrid 28049,Spain [3]Departamento de Biologia,Geologia,Fisica y Quimica Inorganica,Universidad Rey Juan Carlos,C.Tulipan s/n,Mostoles 28933,Spain [4]Departamento de Botanica y Geologia,Facultad de Farmacia,Universidad de Valencia,Avda.Vicente Andres Estelles s/n,Burjassot 46100,Spain [5]Department of Biology,University of Florida,220 Bartram Hall,Gainesville,FL 32611,Florida,USA [6]Vluchtheuvelstraat 6,Dreumel,BK 6621,The Netherlands [7]Visserskade 10,Hank,GL 4273,The Netherlands [8]Kreuzweiler StraBe 22,Perl-Sinz D-66706,Germany [9]Centro de Investigacion en Biodiversidad y Cambio Global,Universidad Autonoma de Madrid,C.Darwin,2,Madrid 28049,Spain

出　　处：《Journal of Systematics and Evolution》2024年第5期979-992,共14页植物分类学报（英文版）

基　　金：supported by the Spanish Ministry of Economy,Industry and Competitiveness and the Spanish Research Agency of the Ministry of Science and Innovation(grant numbers CGL2016-80772-P,PID2020-115149GB-C21,and PID2020-115149GB-C22);the European Union,Next Generation EU;the National Science Foundation of USA(DEB-1541506).

摘　　要：Currently,a wide range of genomic techniques is available at a relatively affordable price.However,not all of them have been equally explored in bryophyte systematics.In the present study,we apply next-generation sequencing to identify samples that cannot be assigned to a taxon by morphological analysis or by Sanger sequencing methods.These samples correspond to a moss with an enigmatic morphology that has been found throughout Western Europe over the last two decades.They exhibit several anomalies in the gametophyte and,on the rare occasions that they appear,also in the sporophyte.The most significant alterations are related to the shape of the leaves.Morphologically,all specimens correspond to mosses of the genus Lewinskya,and the least modified samples are potentially attributable to the Lewinskya affinis complex.Specimen identifications were first attempted using up to seven molecular markers with no satisfactory results.Thus,we employed data generated from targeted enrichment using the GoFlag 408 flagellate land plant probe set to elucidate their identity.Our results demonstrate that all the enigmatic samples correspond to a single species,L.affinis s.str.This approach provided the necessary resolution to confidently identify these challenging samples and may be a powerful tool for similar cases,especially in bryophytes.

关 键 词：BRYOPHYTA GoFlag 408 Lewinskya affnis ORTHOTRICHACEAE targeted enrichment taxonomy 

分 类 号：Q94[生物学—植物学]