LC-Q-TOF/MS法用于伤科天芍凝胶中化学成分的研究  

Identification of Chemical Constituents in Shangketianshao Gel by LC-Q-TOF/MS

作　　者：杨丽 李孟璇[1,2] 钱梦雨 刘文君 闫明[1,2] 曹亮 章晨峰[1,2] 付娟 王振中[1,2] YANG Li;LI Mengxuan;QIAN Mengyu;LIU Wenjun;YAN Ming;CAO Liang;ZHANG Chenfeng;FU Juan;WANG Zhenzhong(Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;National Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Inteligent Manufacture,Lianyungang 222001,China)

机构地区：[1]江苏康缘药业股份有限公司,连云港222001 [2]中药制药过程控制与智能制造技术全国重点实验室,连云港222001

出　　处：《世界科学技术-中医药现代化》2024年第6期1515-1527,共13页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家中医药管理局中央财政转移支付地方项目-基于重点研究室研究领域的中医药多学科研究能力提升项目:中药提取精制新技术,负责人:肖伟。

摘　　要：目的采用LC-Q-TOF/MS技术对伤科天芍凝胶的化学成分进行鉴定。方法以Agilent Eclipse Plus C18柱(250 mm×4.6 mm,5μm)色谱柱,甲醇-0.1%甲酸水为流动相进行梯度洗脱,流速为1.0 mL·min^(-1),柱温30℃。在正、负离子全扫描模式下采集伤科天芍凝胶成分的质谱数据,结合对照品与文献数据信息进行主成分辨析。结果共鉴别出伤科天芍凝胶中103个化学成分,并对所有化合物的药材来源进行了归属,其中22个化合物来源于赤芍、33个化合物来源于大黄、20个化合物来源于白芷、23个化合物来源于黄柏、14个化合物来源于天花粉,共有成分9个。结论该方法快速鉴定了伤科天芍凝胶中的主要成分,为其质量控制指标和药效物质基础的深入研究提供依据。Objective The study analyzed and identified the components in Shangketianshao Gel by LC-Q-TOF/MS.Methods The analysis was performed on Agilent Eclipse Plus C18(250 mm×4.6 mm,5μm)column was applied with methanol and 0.1%formic acid as mobile phase for gradient elution,flow rate was 1 mL·min^(-1)and column temperature was 30℃.The analytes were determined by positive and negative ion modes with electro-spray ionization source,combined message of standard reference and the literature.Results 103 constituents were identified,all compounds were classified to their medicinal materials derivation.22 compounds from Paeoniae Radix Rubra,33 compounds from Rhei Radix et Rhizoma,20 compounds from Angelicae Dahuricae,23 compounds from Cortex Phellodendr and 14 compounds from Trichosanthes kirilowii Maxim.9 components were jointly owned.Conclusion The study provided a suitable way for Chemical fundamentals and quality control of Shangketianshao Gel and laid a foundation for in depth studies of its pharmacodynamics and the quality control.

关 键 词：伤科天芍 LC-Q-TOF/MS 化学成分 归属 

分 类 号：R284.1[医药卫生—中药学]