一种快速检测环境水中苯硫酚的荧光探针  

A fluorescent probe for rapid detection of thiophenol in environmental water

作　　者：王丹妮 张荣荣 安志昊 姬焕焕 李杭 支春燕 王芹[1] 尚永辉[2] 张晟瑞[1] WANG Danni;ZHANG Rongrong;AN Zhihao;JI Huanhuan;LI Hang;ZHI Chunyan;WANG Qin;SHANG Yonghui;ZHANG Shengrui(Shaanxi Key Laboratory of Catalysis,School of Chemistry and Environment Science,Shaanxi University of Technology,Hanzhong 723000,China;School of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China)

机构地区：[1]陕西省催化基础与应用重点实验室,陕西理工大学化学与环境科学学院,汉中723000 [2]咸阳师范学院化学与化工学院,咸阳712000

出　　处：《分析试验室》2024年第9期1223-1228,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(22177066);陕西基础科学(化学、生物学)研究院基础研究计划青年项目(22JHQ017);陕西高校青年创新团队(2022.94);陕西省大学生创新创业训练计划项目(202310720009)资助。

摘　　要：以噻唑半花菁为荧光团(HD),2,4-二硝基苯磺酸酯为识别基团,合成了一种基于分子内光诱导电荷转移机制的“off-on”型荧光探针HD-B。探针HD-B溶液本身荧光较弱且无色,加入苯硫酚(PhSH)后,探针HD-B与其发生芳香亲核取代反应,反应可迅速达到平衡,反应过程中苯磺酸酯键断裂,释放出荧光团,使得570 nm处的荧光强度增加,溶液也由无色变为橙色。探针HD-B对PhSH有良好的定量检测能力,线性响应范围为0~10μmol/L,检出限为0.06μmol/L,对环境水样中PhSH检测的回收率为94.3%~105.4%。该探针可用于环境水样中PhSH的检测。In this work,an“off-on”fluorescent probe HD-B based on photoinduced electron transfer mechanism was synthesized using thiazole hemicyanine as the fluorophore and 2,4-dinitrobenzenesulfonate as the recognition group.The probe HD-B solution exhibits weak fluorescence emission and is colorless.After adding thiophenol,HD-B undergoes a nucleophilic aromatic substitution reaction,which rapidly reaches equilibrium.In the process of this reaction,the benzenesulfonate bond was cracked,releasing the fluorophore,leading to the increment of fluorescence intensity at 570 nm,and simultaneously the solution color change from colorless to orange.The probe HD-B showed excellent quantitative detection ability for thiophenol,with a linear response in the range of 0-10μmol/L and the detection limit of 0.06μmol/L.Furthermore,the recoveries of thiophenol in environment water samples were 94.3%-105.4%.This probe can be used for the detection of thiophenol in environmental water samples.

关 键 词：荧光探针 苯硫酚 半花菁 环境样品 可视化 

分 类 号：O657.3[理学—分析化学]