基于氧化石墨烯荧光适配体传感器检测长江水体中的磺胺嘧啶  

An aptamer fluorescent biosensor based on graphene oxide for the detection of sulfadiazine in Yangtze River water

作　　者：李佩璇 余璐璐 张景瑞 张林 赵昕 何彦平 LI Peixuan;YU Lulu;ZHANG Jingrui;ZHANG Lin;ZHAO Xin;HE Yanping(College of Chemistry and Materials Science,Anhui Normol University,Wuhu 241000,China)

机构地区：[1]安徽师范大学化学与材料科学学院,芜湖241000

出　　处：《分析试验室》2024年第9期1306-1310,共5页Chinese Journal of Analysis Laboratory

基　　金：安徽高校自然科学基金项目(KJ2020A0070);安徽师范大学博士启动基金(903-751974)资助。

摘　　要：利用荧光素基团(FAM)标记的核酸适配体(FAM-SDZ30-1)为探针,氧化石墨烯(GO)为荧光猝灭剂,构建了一种荧光适配体传感器,用于检测长江水体中磺胺嘧啶(SDZ)残留。当溶液中不存在SDZ时,FAM-SDZ30-1通过π-π堆积作用吸附在GO表面,由于荧光共振能量转移,FAM-SDZ30-1的荧光被GO淬灭;当溶液中存在SDZ时,FAM-SDZ30-1与SDZ结合形成复合物,不能吸附在GO表面,溶液的荧光不变。在优化条件下,该传感器荧光强度与SDZ浓度在25~625 ng/mL范围内呈线性关系,相关系数R2为0.9986,检出限为53.13 ng/mL。该方法有望用于长江水体中磺胺嘧啶残留分析。Using 6-carboxyfluorescein(FAM)-labeled aptamer FAM-SDZ30-1 as the probe and graphene oxide(GO)as the fluorescence quenching agent,an aptamer fluorescence biosensor based on GO was constructed for detecting sulfadiazine(SDZ)residues in Yangtze River water.When the absence of SDZ in the solution,FAM-SDZ30-1was adsorbed on the GO surface byπ-πstacking.Due to the fluorescence resonance energy transfer,the fluorescence of FAM-SDZ30-1 was quenched by GO;When SDZ existed in the solution,FAM-SDZ30-1combined with SDZ to form a complex,which could not be adsorbed on the GO surface,and the fluorescence of the solution remained unchanged.Under the optimal conditions,the fluorescence intensity of the aptasensor showed a linear relationship with the concentration of SDZ in the range of 25-625 ng/mL,with a correlation coefficient R~2 of 0.9986,and the detection limit was 53.13 ng/mL.This method could be applied to detect SDZ residues in Yangtze River water.

关 键 词：磺胺嘧啶 氧化石墨烯 适配体传感器 

分 类 号：O657.319[理学—分析化学]