基于多元质量特性过程能力分析的高效液相色谱-串联质谱法检测猪可食组织中常见精神活性物质及其代谢物  

High-performance liquid chromatography-tandem mass spectrometry method for determination of common psychoactive substances and their metabolites in edible tissues of swine based on multivariate mass characterization process capability analysis

作　　者：张惠峰 范宏 孟繁磊[1] 谭莉 张慧[1] 宋志峰[1] ZHANG Huifeng;FAN Hong;MENG Fanlei;TAN Li;ZHANG Hui;SONG Zhifeng(Institute of Agricultural Quality Standards and Testing Technology,Jilin Academy of Agricultural Sciences,Supervision,Inspectin&Testing Center of Agricultural and Processed Products Quality,Minstry of Agriculture and Rural Affairs(Changchun),Changchun 130033,China)

机构地区：[1]吉林省农业科学院农业质量标准与检测技术研究所,农业农村部农产品及加工品质量检验测试中心(长春),长春130033

出　　处：《分析试验室》2024年第9期1323-1331,共9页Chinese Journal of Analysis Laboratory

基　　金：吉林省科技发展计划项目(YDZJ202102CXJD031);吉林省农业科技创新工程人才基金项目(CXGC2020RCG002);吉林省农业科学院农业质量标准与检测技术研究所发展基金项目(ZBS20Y01)资助。

摘　　要：建立了液相色谱-串联质谱法同时测定猪可食组织中氯丙嗪、异丙嗪、甲苯噻嗪等精神活性物质及其代谢物的方法。通过构建回收率及相对标准偏差的多元质量特性双响应曲面模型进行过程能力分析,对QuEChERS方法进行优化,并结合同位素内标法进行检测。结果表明,在质量浓度0.40~200 ng/mL范围内7种目标化合物呈良好的线性关系,相关系数(r^(2))均大于0.999,方法检出限范围为0.05~0.10μg/kg,定量限范围为0.20~0.40μg/kg。5种不同基质中7种目标化合物的加标回收率在78.5%~109%之间,相对标准偏差(RSD)在0.89%~10.8%之间,10日内方法重现性RSD在2.3%~12%之间。所建立的方法适用于猪可食组织中几种常见精神活性物质及其代谢物的定量分析检测。A method for the determination of psychoactive substances and their metabolites such as chlorpromazine,promethazine and xylazine in edible tissues of swine was developed by using high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).The pre-treatment method of QuEChERS was optimized by constructing a multivariate mass characteristic double response surface model of recovery and relative standard deviation(RSD)for process capability evaluation,and the isotope internal standard method was used simultaneously for sample detection.The results showed that good linear relationships with the correlation coefficients(r^(2))greater than 0.999 in the mass concentration range of 0.40-200 ng/mL can be achieved for seven target compounds.This method exhibits excellent detection performance with low limit of detection(LODs)of 0.05-0.10μg/kg and the limit of quantification(LOQs)of 0.20-0.40μg/kg.The recoveries of the seven target compounds in five different matrices were 78.5%-109%,with the relative standard deviations(RSDs)of 0.89%-10.8%.Besides,the reproducibility of this assay within 10 d ranged from 2.3%to 12%.The proposed method is suitable for the quantitative analysis of several common psychoactive substances and their metabolites in the edible tissues of swine.

关 键 词：多元质量特性 过程能力分析 高效液相色谱-串联质谱法 精神活性物质 代谢物 

分 类 号：S859.84[农业科学—临床兽医学] O657.63[农业科学—兽医学]