聚乙烯亚胺荧光增敏法快速高灵敏检测金霉素  

A polyethyleneimine-based fluorescence sensitization strategy for the rapid and highly sensitive detection of chlortetracycline

作　　者：钱美汝 王安琪[1,2] 戴晶 韩建刚 李威[1,2] 李胎花 QIAN Meiru;WANG Anqi;DAI Jing;HAN Jiangang;LI Wei;LI Taihua(Co-Innovation Center for the Sustainable Forestry in Southern China,College of Ecology and Environment,Nanjing Forestry University,Nanjing 210037,China;National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province,Hongze 223100,China)

机构地区：[1]南京林业大学生态与环境学院南方现代林业协同创新中心,南京210037 [2]江苏洪泽湖湿地生态系统国家定位观测研究站,洪泽223100

出　　处：《分析试验室》2024年第9期1332-1337,共6页Chinese Journal of Analysis Laboratory

基　　金：江苏省自然科学基金(BK20191387)项目资助。

摘　　要：在弱酸性环境中利用聚乙烯亚胺(PEI)可对金霉素(CTC)荧光增敏的特性,建立了一种简便快速、高灵敏检测水体和牛奶中痕量CTC的荧光分析方法。在最优条件下,该体系对CTC的定量线性范围为0.005~10μmol/L,检出限为2.7 nmol/L。在实际水体和牛奶中检测CTC的回收率均在88.7%~99.7%之间。该体系在分析实际样品中CTC残留方面具有很好的应用前景。A simple,rapid and highly sensitive fluorescence analysis method for the detection of trace chlortetracycline(CTC)in water and milk was established by using polyethyleneimine(PEI)as a fluorescence sensitizer.Under the optimal conditions,the detection limit of the method was 2.7 nmol/L for CTC in a quantitation linear range of 0.005-10μmol/L.Moreover,the recoveries of CTC in actual water and milk samples were 88.7%-99.7%.The proposed method has a good performance in analysis of CTC residue in real samples.

关 键 词：金霉素 荧光增敏法 聚乙烯亚胺 快速检测 高灵敏 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学] TS252.7[理学—化学]