四川省几种重要猪腹泻病毒分子流行病学调查及其变异性分析  

Molecular epidemiological investigation and variability analysis of several important porcine diarrhea viruses in Sichuan Province

作　　者：夏学妹 陈弟诗[2] 王一丹 向华 植玉鹏 田珺劼 任玉鹏 XIA Xuemei;CHEN Dishi;WANG Yidan;XIANG Hua;ZHI Yupeng;TIAN Junjie;REN Yupeng(College of Animal Husbandry and Veterinary Medicine,Southwest University for Nationalities,Chengdu 61004l,China;Sichuan Provincial Center for Animal Disease Prevention and Control,Chengdu 61004l,China)

机构地区：[1]西南民族大学畜牧兽医学院,四川成都610041 [2]四川省动物疫病预防控制中心,四川成都610041

出　　处：《中国兽医学报》2024年第6期1087-1098,共12页Chinese Journal of Veterinary Science

基　　金：中央高校基本科研业务费专项资金资助项目(2023NYXXS113);四川省科技计划重点研发资助项目“外种猪种源疫病防控关键技术创新研究与示范”(2023YFN0021)。

摘　　要：为了解四川地区流行的猪腹泻病毒类型及其分子流行病学特征,本研究用荧光定量PCR对2021—2023年四川省多个地区来源的猪腹泻样本进行检测;用RT-PCR对猪流行性腹泻病毒(PEDV)、猪A群轮状病毒(PoRVA)、猪德尔塔冠状病毒(PDCoV)和猪捷申病毒(PTV)进行分型鉴定,并分析其基因变异性、遗传进化特征和重组事件。结果显示,PEDV、PoRVA、PDCoV和PTV在四川地区仍呈现流行趋势,其总体阳性率分别为:14.2%(40/281)、13.2%(37/281)、15.6%(44/281)和12.5%(35/281),以PEDV与其他病原混合感染最为常见。本次检测共获得6株G2b型PEDV、3株G3型PDCoV、3株G9P[13]型PoRVA、1株G3P[13]型PoRVA、3株5型PTV、1株9型PTV。6株PEDV毒株与CV777、DR13、KPEDV-9、Chinju99、KNU-0801、AJ1102和LW/L等7个疫苗毒株相比在COE区和S1D中和表位区存在多个氨基酸突变位点;其中PSCLZ01和PSCMY04株在进化树中单独形成一个小支。3株PDCoV与四川往年流行毒株的遗传进化距离较近,但与其相比存在6~48个氨基酸的突变。4株PoRVA与早期疫苗株LLR相比,VP4基因有104~108个氨基酸变异,VP7基因有25个共同的氨基酸变异;从进化树上看,RSCMY01/G3P[13]的VP7基因与黑龙江毒株LNCY同属一个分支,但其VP4基因又与四川SCYA-C7聚为一支,表明该株PoRVA可能是在不同基因型毒株跨省际间传播过程中发生了基因重配。值得注意的是,本次检出PTV-5型和PTV-9型样本中,其他腹泻病毒均为阴性,表明上述两种基因型PTV可能是猪腹泻的重要病原。此外,PEDV PSCLZ01株和PDCoV PCSCMY02株的S基因都发生了重组事件,且亲本毒株来自国内外不同地区。上述结果揭示了近年四川地区流行的主要猪腹泻病毒类型及其基因多样性和遗传变异规律,丰富了四川地区猪腹泻病原的分子流行病学资料,为本地区猪腹泻病的防控和净化提供了重要的理论依据。To investigate the recent prevalence and molecular epidemiological characteristics of porcine diarrhea viruses in Sichuan Province,this study used fluorescence quantitative PCR to detect porcine diarrhea samples from multiple regions in Sichuan Province from 2021 to 2023.RT-PCR was used to identify the genotypes of PEDV,PoRVA,PDCoV,and PTV,and their genetic variability,evolutionary characteristics,and recombination events were analyzed.The results showed that PEDV,PoRVA,PDCoV,and PTV were still prevalent in Sichuan region,with overall positive rates of 14,2%(40/281),13.2%(37/281),15.6%(44/281),and 12.5%(35/281)respectively.PEDV mixed infection with other pathogens was the most common.This study obtained a total of six strains of G2b PEDV,three strains of G3 PDCoV,three strains of G9P[13]PoRVA,one strain of G3P[13]PoRVA,three strains of Type 5 PTV,and one strain of Type 9 PTV.Compared to the seven vaccine strains including CV777,DR13,KPEDV-9,Chinju99,KNU-0801,AJ1102,and LW/L,the 6 PEDV strains showed multiple amino acid mutation sites in the COE region and SID epitope region.Among them,the strains PSCLZ01 and PSCMY04 formed a separate branch in the phylogenetic tree.The three PDCoV strains have a closer genetic evolution distance to the previously prevalent strains in Sichuan,but they also have 6-48 amino acid mutations compared to them.The four PoRVA strains have 104-108 amino acid variations in the VP4 gene compared to the early vaccine strain LLR,and they have 25 common amino acid variations in the VP7 gene.From the phylogenetic tree,the VP7 gene of RSCMY01/G3P[13]belongs to the same branch as the Heilongjiang strain LNCY,but its VP4 gene clusters with the Sichuan strain SCYA-C7,indicating that this PoRVA strain may have undergone genetic reassortment during inter-provincial transmission between different genotypes.It is worth noting that in the detected samples of PTV-5and PTV-9,other diarrheal viruses tested negative,indicating that these two genotypes of PTV may be important pathogens causing porcine diarrhe

关 键 词：猪腹泻病毒 PEDV PoRVA PDCoV PTV 流行病学调查 

分 类 号：S852.65[农业科学—基础兽医学]